Percutaneous absorption of ( sup 14 C)DDT and ( sup 14 C)benzo(a)pyrene from soil

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Fundamental and Applied Toxicology; (USA)

DOI:https://doi.org/10.1016/0272-0590(90)90037-K· OSTI ID:6142673

Wester, R C; Maibach, H I; Bucks, D A; Sedik, L; Melendres, J; Liao, C; DiZio, S ^[1]

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1. Univ. of California School of Medicine, San Francisco (USA)

The objective was to determine percutaneous absorption of DDT and benzo(a)pyrene in vitro and in vivo from soil into and through skin. Soil (Yolo County 65-California-57-8; 26% sand, 26% clay, 48% silt) was passed through 10-, 20-, and 48-mesh sieves. Soil then retained by 80-mesh was mixed with (14C)-labeled chemical at 10 ppm. Acetone solutions at 10 ppm were prepared for comparative analysis. Human cadaver skin was dermatomed to 500 microns and used in glass diffusion cells with human plasma as the receptor fluid (3 ml/hr flow rate) for a 24-hr skin application time. With acetone vehicle, DDT (18.1 +/- 13.4%) readily penetrated into human skin. Significantly less DDT (1.0 +/- 0.7%) penetrated into human skin from soil. DDT would not partition from human skin into human plasma in the receptor phase (less than 0.1%). With acetone vehicle, benzo(a)pyrene (23.7 +/- 9.7%) readily penetrated into human skin. Significantly less benzo(a)pyrene (1.4 +/- 0.9%) penetrated into human skin from soil. Benzo(a)pyrene would not partition from human skin into human plasma in the receptor phase (less than 0.1%). Substantivity (skin retention) was investigated by applying 14C-labeled chemical to human skin in vitro for only 25 min. After soap and water wash, 16.7 +/- 13.2% of DDT applied in acetone remained absorbed to skin. With soil only 0.25 +/- 0.11% of DDT remained absorbed to skin. After soap and water wash 5.1 +/- 2.1% of benzo(a)pyrene applied in acetone remained absorbed to skin. With soil only 0.14 +/- 0.13% of benzo(a)pyrene remained absorbed to skin.

• OSTI ID: 6142673
• Journal Information: Fundamental and Applied Toxicology; (USA), Vol. 15:3; ISSN 0272-0590
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZOPYRENE
SKIN ABSORPTION
DDT
BIOLOGICAL AVAILABILITY
CARBON 14 COMPOUNDS
IN VITRO
MAN
METABOLISM
SOILS
TRACER TECHNIQUES
ABSORPTION
ANIMALS
AROMATICS
CONDENSED AROMATICS
HYDROCARBONS
INSECTICIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PESTICIDES
PRIMATES
UPTAKE
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques