Dose-dependent variations in blood flow evaluation of canine nerve, nerve graft, tendon, and ligament tissue by the radiolabeled-microsphere technique

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Journal of Orthopaedic Research (New York); (USA)

DOI:https://doi.org/10.1002/jor.1100080617· OSTI ID:6142300

Riggi, K; Wood, M B; Ilstrup, D M ^[1]

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1. Mayo Clinic, Rochester, MN (USA)

This study evaluates the dose-dependent accuracy of the radionuclide-labeled microsphere technique for blood flow evaluation in nerve, tendon, and ligament. In eight dogs, blood flows were determined for nerve, nerve graft, tendon, and ligament tissue by simultaneous injection of high- and low-dose microspheres with different radiolabels. The results demonstrated no significant differences in blood flow as measured from the small number of microspheres (less than 400) and the high number (more than 400) for nerve and tendon tissue. For nerve tissue, microsphere counts of 50 to 100, 100 to 200, 200 to 300, and more than 300 produced mean percentage errors of 12.74% (n = 5, SEM = 4.52), 5.45% (n = 13, SEM = 1.22), 10.22% (n = 6, SEM = 4.37), and 17.08% (n = 12, SEM = 3.30), respectively. For tendon tissue, the same microsphere subdivisions had mean percentage errors of 7.47% (n = 4, SEM = 2.66), 3.63% (n = 6, SEM = 1.34), 15.54% (n = 4, SEM = 4.43), and 12.91% (n = 1), respectively. For ligament tissue, percentage errors were consistently higher; microsphere counts of 30 to 100, 100 to 200, and 200 to 300 produced mean errors of 20.14% (n = 4, SEM = 6.38), 18.66% (n = 4, SEM = 6.24), and 25.78% (n = 2, SEM = 1.97), respectively. Although there was no direct relationship between percentage error and number of microspheres retrieved, we suggest that microsphere counts in the range of 100 to 200 should be considered acceptable for nerve and tendon in the canine. Ligament tissue seems to be less well suited to the microsphere technique; however, further study is warranted.

• OSTI ID: 6142300
• Journal Information: Journal of Orthopaedic Research (New York); (USA), Vol. 8:6; ISSN 0736-0266
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LIGAMENTS
BLOOD FLOW
NERVES
ACCURACY
DATA COVARIANCES
DOGS
DOSE-RESPONSE RELATIONSHIPS
MICROSPHERES
STRONTIUM ISOTOPES
ALKALINE EARTH ISOTOPES
ANIMAL TISSUES
ANIMALS
BODY
CONNECTIVE TISSUE
ISOTOPES
MAMMALS
NERVOUS SYSTEM
TISSUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics