Receptor binding sites for substance P in surgical specimens obtained from patients with ulcerative colitis and Crohn disease

Journal Article · Sun May 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.9.3235· OSTI ID:6142248

Mantyh, C R; Gates, T S; Zimmerman, R P; Welton, M L; Passaro, Jr, E P; Vigna, S R; Maggio, J E; Kruger, L; Mantyh, P W

Several lines of evidence indicate that tachykinin neuropeptides (substance P (SP), substance K (SK), and neuromedin K (NK)) play a role in regulating the inflammatory and immune responses. To test this hypothesis in a human inflammatory disease, quantitative receptor autoradiography was used to examine possible abnormalities in tachykinin binding sites in surgical specimens from patients with inflammatory bowel disease. In all cases, specimens were processed for quantitative receptor autoradiography by using /sup 125/I-labeled Bolton-Hunter conjugates of NK, SK, and SP. In colon tissue obtained from ulcerative colitis and Crohn disease patients, very high concentrations of SP receptor binding sites are expressed by arterioles and venules located in the submucosa, muscalairs mucosa, external circular muscle, external longitudinal muscle, and serosa, in contrast to control patients. These results demonstrate that receptor binding sites for SP, but not SK or NK, are ectopically expressed in high concentrations by cells involved in mediating inflammatory and immune responses. These data suggest that SP may be involved in the pathophysiology of inflammatory bowel disease and might provide some insight into the interaction between the nervous system and the regulation of inflammation and the immune response in human inflammatory disease.

• Research Organization: Veterans Administration Wadsworth Medical Center, Los Angeles, CA (USA)
• OSTI ID: 6142248
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:9
• Country of Publication: United States
• Language: English

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DIGESTIVE SYSTEM DISEASES
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LARGE INTESTINE
AUTORADIOGRAPHY
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ARTERIES
INFLAMMATION
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GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
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IODINE ISOTOPES
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LABELLED COMPOUNDS
LYMPHATIC SYSTEM
MEMBRANE PROTEINS
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