BSC-1 growth inhibitor transforms a mitogenic stimulus into a hypertrophic stimulus for renal proximal tubular cells: relationship to Na/sup +//H/sup +/ antiport activity
Renal hypertrophy is characterized by an increase in cell size and protein content with minimal hyperplasia. The mechanisms of control of this pattern of cell growth have not been determined. The present studies examined whether the growth inhibitor elaborated by BSC-1 kidney epilethal cells (GI), which has nearly identical biological properties to transforming growth factor ..beta.. (TGF-..beta..), could transform a mitogenic stimulus into a hypertrophic stimulus for rabbit renal proximal tubular cells in primary culture. Insulin plus hydrocortisone increased the amount of protein per cell, cell volume, and (/sup 3/H)thymidine incorporation at 24 and 48 hr in these cells. When added together with insulin plus hydrocortisone, GI/TGF-..beta.. inhibited the stimulatory effect of these mitogens on (/sup 3/H)thymidine incorporation but did not block the increase in protein per cell and cell volume - i.e., the cells underwent hypertrophy. The fact that this pattern persisted for 48 hr indicated that GI/TGF-..beta.. exerted a prolonged inhibitory effect on mitogenic-stimulated DNA synthesis rather than delaying its onset. Amiloride-sensitive Na/sup +/ uptake using /sup 22/Na/sup +/ as a tracer, correlated with protein per cell and cell volume rather than with DNA synthesis. These studies indicate that the control of cell size may be regulated by autocrine mechanisms mediated by the elaboration of growth inhibitory factors that alter the pattern of the growth response to mitogens.
- Research Organization:
- Univ. of California, Los Angeles, CA (United States)
- OSTI ID:
- 6138351
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:18
- Country of Publication:
- United States
- Language:
- English
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ANIMAL GROWTH
INHIBITION
KIDNEYS
HYPERTROPHY
PROTONS
MEMBRANE TRANSPORT
SODIUM 22
SODIUM CHLORIDES
ANIMAL CELLS
CATIONS
DNA REPLICATION
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
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BARYONS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
ELEMENTARY PARTICLES
FERMIONS
GROWTH
HADRONS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEONS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PYRIMIDINES
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YEARS LIVING RADIOISOTOPES
550301* - Cytology- Tracer Techniques
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