Isolation of pigmentation mutants of the green filamentous photosynthetic bacterium Chloroflexus aurantiacus

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · J. Bacteriol.; (United States)

OSTI ID:6135167

Pierson, B K; Keith, L M; Leovy, J G

Mutants deficient in the production of bateriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus, Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were deficient in Bchl c synthesis. All reverted. One double mutant deficient both in Bchl c synthesis and in the synthesis of colored carotenoids under anaerobic conditions was isolated. Isolation of a revertant in Bchl c synthesis from this double mutant produced a mutant strain of Chloroflexus that grew photosynthetically under anaerobic conditions and lacked colored carotenoids. Analysis of pigment contents and growth rates of the mutants revealed a positive association between growth rate and content of Bchl c under light-limiting conditions. 11 references, 4 figures, 3 tables.

• Research Organization: Univ. of Puget Sound, Tacoma, WA
• OSTI ID: 6135167
• Journal Information: J. Bacteriol.; (United States), Vol. 159:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NITRO COMPOUNDS
GENETIC EFFECTS
PHOTOSYNTHETIC BACTERIA
GENETIC RADIATION EFFECTS
GROWTH
CHLOROPHYLL
MUTAGENS
MUTANTS
MUTATIONS
PIGMENTS
REVERTANTS
TOXICITY
ULTRAVIOLET RADIATION
VISIBLE RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PORPHYRINS
RADIATION EFFECTS
RADIATIONS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)