1-alkenyl-2-acyl glycerol is an intermediate in myocardial plasmenylcholine biosynthesis

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6125901

Ford, D; Gross, R

The present study was undertaken to identify the metabolic pathway(s) responsible for myocardial plasmenylcholine biosynthesis. Rabbit myocardium contained .46 +/- .09 nmol/g wet wight of 1-alkenyl-2-acyl glycerol (AAG) which predominantly consisted of 16:0 molecular species at the sn-1 position. Incubation of rabbit myocardial microsomes (RMM) with (/sup 14/C)CDP-choline (/sup 14/C-CDPC) resulted in the rapid incorporation of radiolabeled choline into the choline glycerophospholipid pool. RP-HPLC separation of molecular species demonstrated that nearly equal amounts of radiolabel were incorporated into plasmenylcholine and phosphatidylcholine subclasses despite the fact that RMM contained 21 times the mass of diacyl glycerol as compared to AAG. RMM incorporation of /sup 14/C-CDPC into choline glycerophospholipids was substantially greater than incorporation of (/sup 14/C) phosphorylcholine or (/sup 14/C) choline. RMM incorporation of /sup 14/C-CDPC into plasmalogen molecular species was stimulated two fold by 500 ..mu..M CMP. Taken together, these results demonstrate that rabbit myocardium contains substantial quantities of AAG and that endogenous AAG is an efficient precursor of myocardial plasmenylcholine.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 6125901
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLINE
BIOSYNTHESIS
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
GLYCEROL
LIQUID COLUMN CHROMATOGRAPHY
MICROSOMES
MYOCARDIUM
RABBITS
TRACER TECHNIQUES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHROMATOGRAPHY
DRUGS
HEART
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MUSCLES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
QUATERNARY COMPOUNDS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques