Leukocyte labeling with technetium-99m tin colloids
Triple density gradients of metrizamide in plasma (MP) were used to characterize label distribution in human leukocyte preparations incubated with /sup 99m/Tc tin colloids. Less than 50% of the cell-associated radioactivity was specifically bound to leukocytes when heparinized blood was rotated with stannous fluoride colloid ((Tc)SFC). Labeling efficiency in leukocyte rich plasma (LRP) averaged 44%, of which greater than 90% was specifically bound to leukocytes. MP-gradient analysis also revealed that leukocyte labeling did not occur with stannous chloride colloid, nor when citrate was present during rotation with (Tc)SFC. When citrate was added after labeling to solubilize unbound (Tc)SFC, radiocolloid was removed from the leukocytes, indicating that the mechanism of (Tc)SFC labeling is adherence rather than phagocytosis. Technetium-labeled neutrophils exhibited normal in vitro chemotaxis and no lung uptake in vivo. Technetium-labeled mononuclear leukocytes, on the other hand, exhibited prolonged lung transit in vivo. Neither (Tc)SFC cell preparation showed signs of in vivo reoxidation to pertechnetate.
- Research Organization:
- Indiana Univ. Medical Center, Indianapolis
- OSTI ID:
- 6125139
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 28:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LEUKOCYTES
LABELLING
ADHESION
ISOMERIC NUCLEI
MAN
RADIOCOLLOIDS
RADIOPHARMACEUTICALS
TECHNETIUM 99
TIN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COLLOIDS
DISPERSIONS
DRUGS
ELEMENTS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METALS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics