Transformation and characterization of bovine endothelial cells after in vitro treatment with benzo(a)pyrene
A cloned strain of bovine vascular endothelial cells with a finite in vitro lifespan was treated with the chemical carcinogen benzo(a)pyrene. Untreated cultures of this cell line senesced upon serial subcultivation and contained large non-dividing cells. In four out of eight trials, repeated subcultivation in the presence of benzo(a)pyrene produced transformed lines which appeared in cultures concomitant with the senescence of the parent cells. These lines exhibited an indefinite lifespan and a variety of altered phenotypic characteristics. Four characterized cell lines fit into two general categories. Two lines acquired an indefinite lifespan but otherwise exhibited characteristics of normal endothelial cells: contact inhibition of growth, anchorage dependence, serum requirement for growth of at least 5% fetal bovine serum, and inability to produce tumors in syngeneic hosts. In the other category, two lines acquired those characteristics normally associated with transformation: lack of contact inhibition of growth, anchorage independence, and ability to form tumors (hemangiosarcomas) in syngeneic hosts. Transformed lines lost some specialized characteristics retained by normal endothelial cells in vitro, although the ability to form hemangiosarcomas was retained by the tumorigenic cells. Finally, the two tumorigenic transformed lines have been found to contain non-random chromosomal changes: translocations of chromosome 15 and 21 and trisomy of chromosome 15. Preliminary studies have related these changes to the tumorigenicity of the cells because a non-tumorigenic line does not contain them and these alterations have also been demonstrated in the tumors caused by the injection of the tumorigenic cells.
- OSTI ID:
- 6109016
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CULTURES
COMPARATIVE EVALUATIONS
NEOPLASMS
TUMOR CELLS
ANIMAL CELLS
AROMATICS
CONDENSED AROMATICS
DISEASES
HYDROCARBONS
ORGANIC COMPOUNDS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)