Monoclonal antibodies specific for sickle cell hemoglobin
Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility. 12 references, 4 figures.
- Research Organization:
- Lawerence Livermore National Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6105446
- Journal Information:
- Hemoglobin; (United States), Vol. 9:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMOGLOBIN
IMMUNE REACTIONS
SCREENING
MONOCLONAL ANTIBODIES
BIOCHEMICAL REACTION KINETICS
AMINO ACIDS
CELL FLOW SYSTEMS
ELECTROPHORESIS
HYBRIDOMAS
MICE
PEPTIDES
SICKLE CELL ANEMIA
ANEMIAS
ANIMAL CELLS
ANIMALS
ANTIBODIES
CARBOXYLIC ACIDS
DISEASES
GLOBIN
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
RODENTS
SYMPTOMS
VERTEBRATES
550200* - Biochemistry