Investigations on the transport and metabolism of high density lipoprotein cholesteryl esters in African green monkeys
The metabolic fate of circulating high density lipoprotein cholesteryl esters was studied in African green monkeys to determine the significance of the lipid transfer reaction on the catabolism of lipoprotein cholesteryl esters. A method of doubly labeling both moieties of lipoprotein cholesteryl esters with (/sup 3/He)cholesteryl oleate and cholesteryl (/sup 14/C)oleate was developed for the purpose of studying plasma cholesteryl ester metabolism in vivo. In these studies the total plasma (/sup 3/He)cholesterol turnover resulted in production rates, which ranged from 10-17 mg/kg day, similar to previously reported values in African green monkeys and in normal lipoproteinemic humans. In contrast to the production rates calculated from the decay of plasma /sup 3/He-radioactivity, the production rates calculated from lipoproteins labeled with cholesteryl (/sup 14/C)oleate were approximately 2-3 times greater. In addition to these studies, a plasma cholesteryl ester transacylation activity was demonstrated in vitro when HDL containing doubly labeled cholesteryl esters were incubated with fresh plasma. These results demonstrated that high density lipoprotein cholesteryl esters undergo transacylation in vitro, resulting in release and reesterification of free (/sup 3/H)cholesterol.
- Research Organization:
- Wake Forest Univ., Winston-Salem, NC (USA)
- OSTI ID:
- 6105170
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14 COMPOUNDS
METABOLISM
LIPOPROTEINS
TRITIUM COMPOUNDS
ACYLATION
CHOLESTEROL
ESTERS
MEMBRANE TRANSPORT
MONKEYS
OLEIC ACID
ANIMALS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
STEROIDS
STEROLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques