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Title: Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles

Abstract

This paper describes a scheme for differential identification of Candida species and other yeasts based on autoradiographic analysis of protein profiles of (/sup 35/S)methionine-labeled cellular proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using ATCC strains as references, protein profile analysis showed that different Candida and other yeast species produced distinctively different patterns. Good agreement in results obtained with this approach and with other conventional systems was observed. Being accurate and reproducible, this approach provides a basis for the development of an alternative method for the identification of yeasts isolated from clinical specimens.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Veterans General Hospital, Taipei, Taiwan
OSTI Identifier:
6101683
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 175:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; POLYPEPTIDES; AUTORADIOGRAPHY; YEASTS; CLASSIFICATION; CANDIDA; ELECTROPHORESIS; METHIONINE; MOLECULAR WEIGHT; SULFUR 35; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; EUMYCOTA; EVEN-ODD NUCLEI; FUNGI; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MICROORGANISMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PEPTIDES; PLANTS; PROTEINS; RADIOISOTOPES; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques; 550701 - Microbiology- Tracer Techniques

Citation Formats

Shen, H D, Choo, K B, Tsai, W C, Jen, T M, Yeh, J Y, and Han, S H. Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles. United States: N. p., 1988. Web. doi:10.1016/0003-2697(88)90582-9.
Shen, H D, Choo, K B, Tsai, W C, Jen, T M, Yeh, J Y, & Han, S H. Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles. United States. https://doi.org/10.1016/0003-2697(88)90582-9
Shen, H D, Choo, K B, Tsai, W C, Jen, T M, Yeh, J Y, and Han, S H. 1988. "Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles". United States. https://doi.org/10.1016/0003-2697(88)90582-9.
@article{osti_6101683,
title = {Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles},
author = {Shen, H D and Choo, K B and Tsai, W C and Jen, T M and Yeh, J Y and Han, S H},
abstractNote = {This paper describes a scheme for differential identification of Candida species and other yeasts based on autoradiographic analysis of protein profiles of (/sup 35/S)methionine-labeled cellular proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using ATCC strains as references, protein profile analysis showed that different Candida and other yeast species produced distinctively different patterns. Good agreement in results obtained with this approach and with other conventional systems was observed. Being accurate and reproducible, this approach provides a basis for the development of an alternative method for the identification of yeasts isolated from clinical specimens.},
doi = {10.1016/0003-2697(88)90582-9},
url = {https://www.osti.gov/biblio/6101683}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 175:2,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 1988},
month = {Thu Dec 01 00:00:00 EST 1988}
}