Thiamine metabolism in folate deficient rats
Folate status (FS) and resultant alterations in thiamine status (TS) were evaluated in weanling rats fed either 17% amino acids (RHAA); 14% amino acids (LOGLU); 20% Vitamin Free casein (VFC) + 8% gelatin (HICG); 10% VFC + 4% gelatin + 0.3% methionine (CGM); or 10% VFC + 4 % gelatin (LOCG). Diets were fed with and without 8 mg FA/kg diet. HICG diet contained 54 ug/kg endogenous folate, the CGM and LOCG, 27 ug/kg, RHAA and LOGLU were folate free. FS was assessed by growth rate, hematology, formiminoglutamic acid excretion following a histidine load and tissue folate levels. TS was assessed by determining the fate of oral /sup 3/H-labeled and intravenous /sup 14/C-labeled thiamine over a six hour test period and by measurement of blood transketolase activity (TKA) and TPP effect (TPPE). TKA and TPPE were measured by an enzymatic single-point assay developed during these investigations.
- Research Organization:
- California Univ., Davis (USA)
- OSTI ID:
- 6100777
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FOLIC ACID
NUTRITIONAL DEFICIENCY
THIAMINE
METABOLISM
TRANSFERASES
ENZYME ACTIVITY
ANIMAL GROWTH
CARBON 14 COMPOUNDS
DIET
METHIONINE
RADIOENZYMATIC ASSAY
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
AZINES
AZOLES
CARBOXYLIC ACIDS
DRUGS
ENZYMES
GROWTH
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PTERIDINES
PYRIMIDINES
RODENTS
THIAZOLES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550501* - Metabolism- Tracer Techniques