Plasma membrane reorganization induced by tumor promoters in an epithelial cell line

Packard, B S; Saxton, M J; Bissell, M J; Klein, M P

doi:10.1073/pnas.81.2.449

Title: Plasma membrane reorganization induced by tumor promoters in an epithelial cell line

Full Record
Other Related Research

Abstract

The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters (percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)) connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding upon the interaction of cells with tumor promoters. The idea of induced reorganization is supported by experiments in which cell shape change, brought about by either exposure to cytochalasin B or growth on matrices of collagen, fibronectin, or laminin, resulted in values in the fluorescence recovery after photobleaching technique similar to those with active phorbol esters. 35 references, 3 figures, 1 table.

Authors:: Packard, B S; Saxton, M J; Bissell, M J; Klein, M P

Publication Date:: Sun Jan 01 00:00:00 EST 1984

Research Org.:: Univ. of California, Berkeley, CA (United States)

OSTI Identifier:: 6098806

DOE Contract Number:: AC03-76SF00098; AT03-80ER10700

Resource Type:: Journal Article

Journal Name:: Proc. Natl. Acad. Sci. U.S.A.; (United States)

Additional Journal Information:: Journal Volume: 81:1

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; MORPHOLOGICAL CHANGES; ONCOGENIC TRANSFORMATIONS; BIOLOGICAL MODELS; PHORBOL ESTERS; BIOLOGICAL EFFECTS; CELL CULTURES; CELL MEMBRANES; DIFFUSION; EPITHELIUM; FLUORESCENCE; LIPIDS; ANIMAL TISSUES; BODY; CARCINOGENS; CELL CONSTITUENTS; ESTERS; LUMINESCENCE; MEMBRANES; ORGANIC COMPOUNDS; TISSUES; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)

Citation Formats


                    Packard, B S, Saxton, M J, Bissell, M J, and Klein, M P. Plasma membrane reorganization induced by tumor promoters in an epithelial cell line.  United States: N. p., 1984. 
        Web.  doi:10.1073/pnas.81.2.449.

Copy to clipboard


                    Packard, B S, Saxton, M J, Bissell, M J, & Klein, M P. Plasma membrane reorganization induced by tumor promoters in an epithelial cell line.  United States.  https://doi.org/10.1073/pnas.81.2.449

Copy to clipboard


                    Packard, B S, Saxton, M J, Bissell, M J, and Klein, M P. 1984.  
        "Plasma membrane reorganization induced by tumor promoters in an epithelial cell line".  United States.  https://doi.org/10.1073/pnas.81.2.449.

Copy to clipboard


                    
@article{osti_6098806,

  title        = {Plasma membrane reorganization induced by tumor promoters in an epithelial cell line},

  author       = {Packard, B S and Saxton, M J and Bissell, M J and Klein, M P},

  abstractNote = {The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters (percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)) connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding upon the interaction of cells with tumor promoters. The idea of induced reorganization is supported by experiments in which cell shape change, brought about by either exposure to cytochalasin B or growth on matrices of collagen, fibronectin, or laminin, resulted in values in the fluorescence recovery after photobleaching technique similar to those with active phorbol esters. 35 references, 3 figures, 1 table.},

  doi          = {10.1073/pnas.81.2.449},

  url          = {https://www.osti.gov/biblio/6098806},
  journal      = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number       = ,

  volume       = 81:1,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 1984},

  month        = {Sun Jan 01 00:00:00 EST 1984}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1073/pnas.81.2.449

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Plasma membrane reorganization induced by tumor promoters in an epithelial cell line

Journal Article PACKARD, BEVERLY; SAXTON, MICHAEL; BISSELL, MINA; ... - Proceedings of the National Academy of Science

The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters [percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)] connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding uponmore »« less
https://doi.org/10.1073/pnas.81.2.449

Full Text Available
Plasma membrane reorganization induced by chemical transformation in cultura

Technical Report Packard, B

Induction of increased rigidity in the plasma membrane paralleling properties associated with a transformed state was suggested by two experiments. Fluorescence recovery after photobleaching (FRAP) indicated the induction of an environment in the plasma membrane where the synthetic fluorescent phospholipid collarein was immobile on the FRAP timescale. The other technique revealed the binding of epidermal growth factor (EGF) to a cryptic class of receptors which become accessible upon chemical transformation. These two lines of evidence are consistent with a reorganization of the plasma membrane induced by tumor promoters. 110 references, 38 figures, 4 tables.
12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells

Journal Article Shiba, Yoshiki; Sasaki, Yasuto; Kanno, Yoshinobu - Experimental Cell Research; (United States)

The effect of a tumor-promoting phorbol ester on the binding of fibronectin-coated beads to 3T3-L1 cells was studied to clarify the relationship between the binding of fibronectin to the cells, cell adhesion, and the organization of actin filaments. Interference-reflection microscopy revealed focal contacts of 3T3-L1 cells with the substratum. Stress fibers observed after rhodomine-phalloidin staining were well-developed in the cells. Treatment of the cells for 20 min with 12-O-tetradecanoylphorbol-13-acetate (TPA), but not with phorbol, disrupted focal contacts and caused a reorganization of stress fibers to generate actin ribbons. Treatment of the cells with TPA enhanced the binding of beads coatedmore »« less
https://doi.org/10.1016/0014-4827(88)90394-1
Phorbol esters enhance attachment of NIH/3T3 cells to laminin and type IV collagen substrates

Journal Article Kato, Shigemi; Ben, T; De Luca, L - Experimental Cell Research; (United States)

The effect of phorbol esters on the adhesive properties of NIH/3T3 mouse fibroblasts was investigated using plastic substrates precoated with the extracellular matrix proteins fibronectin, collagen, and laminin. Treatment with phorbol 12-myristate 13-acetate (PMA) enhanced NIH/3T3 cell attachment to laminin and type IV collagen substrates but had little or no effect on attachment to fibronectin and type I collagen substrates. The effect of PMA in enhancing cell attachment to laminin and type IV collagen substrates was dose dependent between 10{sup {minus}9} and 10{sup {minus}7} M. PMA was effective as early as 30 min; the effect reached a maximum at 2more »« less
https://doi.org/10.1016/0014-4827(88)90345-X
The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating

Journal Article Pajęcka, Kamilla; Nielsen, Malik; Hansen, Troels; ... - Experimental Cell Research

Background and aims: Nephropathy involves pathophysiological changes to the glomerulus. The primary glomerular endothelial cells (GEnCs) have emerged as an important tool for studying glomerulosclerotic mechanisms and in the screening process for drug-candidates. The success of the studies is dependent on the quality of the cell model. Therefore, we set out to establish an easy, reproducible model of the quiescent endothelial monolayer with the use of commercially available extracellular matrices (ECMs). Methods: Primary hGEnCs were seeded on various ECMs. Cell adhesion was monitored by an impedance sensing system. The localization of junctional proteins was assessed by immunofluorescence and the barriermore »« less
https://doi.org/10.1016/J.YEXCR.2017.02.039

Similar Records