Effects of hyperthermia and radiation in two L5178Y cell lines with different efficiencies of DNA repair
L5178Y-R and L5178Y-S cells show inverse cross-sensitivity to X-rays and UV light. Recovery of cells after exposure to X-rays, DNA single-strand break rejoining, and excision repair are more efficient in L5178Y-R than in L5178Y-S cells. Caffeine-inhibitable, postreplication repair of UV-induced damage is present in L5178Y-S but not in L5178Y-R cells. Exposure to heat (41 degrees C) for up to 300 minutes did not affect survival of either cell line. Heating time-survival relationship for 43 degrees C was exponential for L5178Y-R cells (D0.9.1 min), whereas that for L5178Y-S was sigmoid (D0.7.8 min, n.2.5). Sparing effect due to heating fractionation (2X15 min at 43 degrees C separated by different intervals at 37 degrees C, vs. 30 min at 43 degrees C) was much more pronounced in L5178Y-S than in L5178Y-R cells (relative survival 8 and 2, for a 30-min interval, respectively). Preheating at 41 degrees C (120 min) had little effect on X-ray sensitivity of either cell line. Preheating at 43 degrees C (30 min) increased X-ray sensitivity of both cell lines (thermal enhancement ratios: 2.1 and 1.8 for L5178Y-R and L5178Y-S cells, respectively).
- Research Organization:
- Department of Radiobiology and Health Protection, Institute of Nuclear Research, Warszawa-Zeran, Poland
- OSTI ID:
- 6095869
- Journal Information:
- NIDA Res. Monogr.; (United States), Vol. 61
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL CULTURES
GENETIC RADIATION EFFECTS
DNA
BIOLOGICAL REPAIR
COMPARATIVE EVALUATIONS
HYPERTHERMIA
LEUKEMIA
MICE
RESEARCH PROGRAMS
RESPONSE MODIFYING FACTORS
SENSITIVITY
SURVIVAL CURVES
ULTRAVIOLET RADIATION
USA
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BODY TEMPERATURE
DISEASES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
HEMIC DISEASES
IONIZING RADIATIONS
MAMMALS
NEOPLASMS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)