Genotoxicity of alpha particles in human embryonic skin fibroblasts
Cell inactivation and induced mutation frequencies at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus have been measured in cultured human fibroblasts (GM10) exposed to ..cap alpha.. particles from /sup 238/ Pu and 250 kVp X rays. The survival curves resulting from exposure to ..cap alpha.. particles are exponential. The mean lethal dose, D/sub 0/, is approximately 1.3 Gy for X rays and 0.25 Gy for ..cap alpha.. particles. As a function of radiation dose, mutation induction at the HGPRT locus was linear for ..cap alpha.. particles whereas the X-ray-induced mutation data were better fitted by a quadratic function. When mutation frequencies were plotted against the log of survival, mutation frequency at a given survival level was greater in cells exposed to ..cap alpha.. particles than to X rays.
- Research Organization:
- Los Alamos National Lab., NM
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6095645
- Journal Information:
- Radiat. Res.; (United States), Vol. 100:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALPHA PARTICLES
RBE
CELL KILLING
RADIOINDUCTION
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
MUTATIONS
DOSE-RESPONSE RELATIONSHIPS
EMBRYONIC CELLS
EXPERIMENTAL DATA
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MAN
MUTATION FREQUENCY
PLUTONIUM 238
SKIN
SURVIVAL CURVES
X RADIATION
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
DATA
ELECTROMAGNETIC RADIATION
ENZYMES
EVEN-EVEN NUCLEI
GLYCOSYL TRANSFERASES
HEAVY NUCLEI
INFORMATION
IONIZING RADIATIONS
ISOTOPES
MAMMALS
NUCLEI
NUMERICAL DATA
ORGANS
PENTOSYL TRANSFERASES
PLUTONIUM ISOTOPES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)
550401 - Genetics- Tracer Techniques
550301 - Cytology- Tracer Techniques