DNA damage by smoke: Protection by turmeric and other inhibitors of ROS
- Department of Nutrition and Food Safety, Central Food Technological Research Institute, Mysore (India)
Twigs-dry leaves smoke condensate (TDS), as a source of clastogenic ROS and carcinogenic PAH, was investigated for its in vitro DNA-damaging effect in calf thymus DNA and human peripheral lymphocytes. An aqueous turmeric component--Aq.T--with an established antioxidant activity, was tested as a DNA protectant. TDS induced 13-fold damage to calf thymus DNA as judged by the emergence of a DNA damage specific, fluorescent product (em: 405 nm). Aq.T at 800 ng/microL extended 69% protection to calf thymus DNA and was comparable to the other protectants such as curcumin, BHA, vitamin E, SOD, and CAT. In human peripheral lymphocytes, TDS induced extensive DNA damage in comparison with the tumor promoter TPA, as judged by FADU. Aq.T at 300 ng/microL extended 90% protection to human lymphocyte DNA against TDS-induced damage, and was more effective than the other protectants--DABCO, D-mannitol, sodium benzoate, vitamin E (ROS quenchers), SOD, CAT (antioxidant enzymes), tannic acid, flufenamic acid, BHA, BHT, n-PG, curcumin and quercetin (antioxidants). Aq.T offered 65% protection to human lymphocyte DNA against TPA-induced damage and was comparable to SOD. The above results indicate that TDS induces substantial DNA damage in calf thymus DNA and human lymphocytes and Aq.T is an efficient protectant.
- OSTI ID:
- 6093721
- Journal Information:
- Free Radical Biology and Medicine; (United States), Vol. 11:3; ISSN 0891-5849
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIOXIDANTS
BIOLOGICAL EFFECTS
POLYCYCLIC AROMATIC HYDROCARBONS
GENETIC EFFECTS
CALVES
CURCUMIN
DNA
LYMPHOCYTES
MAN
RADICALS
SMOKES
THYMUS
AEROSOLS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CATTLE
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
DOMESTIC ANIMALS
DYES
ETHERS
HYDROCARBONS
HYDROXY COMPOUNDS
KETONES
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PRIMATES
RESIDUES
RUMINANTS
SOLS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology