Rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · J. Lipid Res.; (United States)

OSTI ID:6090213

Khoo, J C; Miller, E; Goldberg, D I

An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with /sup 63/Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.

• Research Organization: Univ. of California, San Diego, La Jolla
• OSTI ID: 6090213
• Journal Information: J. Lipid Res.; (United States), Vol. 28:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RADIOENZYMATIC ASSAY
SENSITIVITY ANALYSIS
TRIGLYCERIDES
ACCURACY
CARBOXYLIC ACIDS
CELL CULTURES
MACROPHAGES
MICE
NICKEL 63
TRACER TECHNIQUES
TRIOLEIN
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CONNECTIVE TISSUE CELLS
ESTERS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MAMMALS
NICKEL ISOTOPES
NUCLEI
OILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PHAGOCYTES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques