Effects of membrane physical parameters on hematoporphyrin-derivative binding to liposomes: a spectroscopic study
Physical parameters of membrane bilayers were studied for their effect on the binding of hematoporphyrin derivative (Hpd), which is used as a sensitizer in photodynamic therapy of cancerous tissues. The purpose of this study was to clarify which parameters were relevant, under physiological conditions, to the selectivity of Hpd binding to cancer cells. Fluorescence spectroscopy was used to measure the relative partitioning of the dye between the lipid and aqueous media. Increasing the microviscosity of the liposomes' membranes by various bilayer additives results in a strong reduction of Hpd binding, to an extent independent of the specific additive. The effect of temperature near the physiological value as well as the effect of cross membrane potential are small. Surface potential does not affect the binding constant, indicating that the binding species does not carry a net electric charge.
- Research Organization:
- Bar-Ilan Univ., Ramat-Gan, Israel
- OSTI ID:
- 6087520
- Journal Information:
- J. Membr. Biol.; (United States), Vol. 97:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
PHYSICAL PROPERTIES
HEMATOPORPHYRINS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
CHOLESTEROL
FLUORESCENCE
LIPOSOMES
RADIOSENSITIZERS
TUMOR CELLS
ANIMAL CELLS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DRUGS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LUMINESCENCE
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PIGMENTS
PORPHYRINS
REACTION KINETICS
STEROIDS
STEROLS
560152* - Radiation Effects on Animals- Animals