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Title: Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation

Abstract

Trypanosoma cruzi epimastigotes (insect gut stage) incubated with (U- UC)glucose synthesized Man9GlcNAc2-P-P-dolichol as practically the sole dolichol-P-P derivative. On the other hand, amastigotes (intracellular stage) of the same parasite synthesized four to five times more Man7GlcNAc2-P-P-dolichol than Man9GlcNAc2-P-P-dolichol. Evidence is presented indicating that, whereas in epimastigotes only Man9GlcNAc2 was transferred to proteins, in amastigotes both Man7GlcNAc2 and Man9GlcNAc2 were transferred in direct proportion to their respective amounts bound to dolichol-P-P. The change in the mechanism of protein N-glycosylation could be observed upon in vitro differentiation of amastigotes to epimastigotes. The dissimilar size of the main oligosaccharides transferred to proteins in epimastigotes and amastigotes was responsible for differences in two structural features of high mannose-type oligosaccharides present in mature glycoproteins of both forms of the parasite, namely the average size of the compounds and the structure of the main species of some isomer oligosaccharides.

Authors:
;
Publication Date:
Research Org.:
Instituto Nacional del Diagnostico e Investigacion de la Enfermedad de Chagas, Buenos Aires, Argentina
OSTI Identifier:
6086066
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; BIOSYNTHESIS; GLUCOPROTEINS; TRYPANOSOMA; CELL DIFFERENTIATION; GLUCOSE; OLIGOSACCHARIDES; ALDEHYDES; CARBOHYDRATES; HEXOSES; LABELLED COMPOUNDS; MASTIGOPHORA; MONOSACCHARIDES; ORGANIC COMPOUNDS; PARASITES; PROTEINS; SACCHARIDES; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Engel, J C, and Parodi, A J. Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation. United States: N. p., 1985. Web.
Engel, J C, & Parodi, A J. Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation. United States.
Engel, J C, and Parodi, A J. 1985. "Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation". United States.
@article{osti_6086066,
title = {Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation},
author = {Engel, J C and Parodi, A J},
abstractNote = {Trypanosoma cruzi epimastigotes (insect gut stage) incubated with (U- UC)glucose synthesized Man9GlcNAc2-P-P-dolichol as practically the sole dolichol-P-P derivative. On the other hand, amastigotes (intracellular stage) of the same parasite synthesized four to five times more Man7GlcNAc2-P-P-dolichol than Man9GlcNAc2-P-P-dolichol. Evidence is presented indicating that, whereas in epimastigotes only Man9GlcNAc2 was transferred to proteins, in amastigotes both Man7GlcNAc2 and Man9GlcNAc2 were transferred in direct proportion to their respective amounts bound to dolichol-P-P. The change in the mechanism of protein N-glycosylation could be observed upon in vitro differentiation of amastigotes to epimastigotes. The dissimilar size of the main oligosaccharides transferred to proteins in epimastigotes and amastigotes was responsible for differences in two structural features of high mannose-type oligosaccharides present in mature glycoproteins of both forms of the parasite, namely the average size of the compounds and the structure of the main species of some isomer oligosaccharides.},
doi = {},
url = {https://www.osti.gov/biblio/6086066}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 18,
place = {United States},
year = {Sun Aug 25 00:00:00 EDT 1985},
month = {Sun Aug 25 00:00:00 EDT 1985}
}