Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium
A serumless, chemically defined medium has been developed for the culture of oligodendrocytes isolated from primary neonatal rat cerebral cultures. Combined together, insulin, transferrin, and fibroblast growth factor synergistically induced an essentially homogeneous population (95-98%) of cells expressing glycerol-3-phosphate dehydrogenase activity to undergo cell division. Proliferating cells were characterized by several criteria: (i) ultrastructural analysis by transmission electron microscopy identified the cell type as an oligodendrocyte; (ii) biochemical assays showed expression of three oligodendrocyte biochemical markers, induction of both glycerol phosphate dehydrogenase and lactate dehydrogenase, and presence of 2',3'-cyclic nucleotide 3'-phosphodiesterase; and (iii) immunocytochemical staining showed cultures to be 95-98% positive for glycerol phosphate dehydrogenase, 90% for myelin basic protein, 60-70% for galactocerebroside, and 70% for A2B5.
- Research Organization:
- Univ. of California, Los Angeles, CA (United States)
- DOE Contract Number:
- AC03-76SF00012
- OSTI ID:
- 6085359
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:10
- Country of Publication:
- United States
- Language:
- English
Similar Records
In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination
Bipotential precursors of putative fibrous astrocytes and oligodendrocytes in rat cerebellar cultures express distinct surface features and neuron-like. gamma. -aminobutyric acid transport
Related Subjects
HEMIACETAL DEHYDROGENASES
BIOCHEMISTRY
LACTATE DEHYDROGENASE
NERVE CELLS
CYTOLOGY
PHOSPHODIESTERASES
CELL PROLIFERATION
CEREBRUM
CULTURE MEDIA
NEONATES
RATS
THYMIDINE
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
ULTRASTRUCTURAL CHANGES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
ELECTRON MICROSCOPY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques