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Title: Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter

Abstract

A recombinant Saccharomyces cerevisiae, expressing and secreting rice [alpha]-amylase, converts starch to ethanol. The rice [alpha]-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N[sub 3]-GCTTCCAA-N[sub 5]-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha, and Candida biodinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch. 45 refs., 8 figs.

Authors:
; ; ;  [1]
  1. Biosource Genetics Corporation, Vacaville, CA (United States)
Publication Date:
OSTI Identifier:
6084916
Resource Type:
Journal Article
Journal Name:
Bio/Technology; (United States)
Additional Journal Information:
Journal Volume: 11:5; Journal ID: ISSN 0733-222X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ETHANOL; BIOSYNTHESIS; SACCHAROMYCES CEREVISIAE; GENE RECOMBINATION; BIOTECHNOLOGY; DNA SEQUENCING; ALCOHOLS; EUMYCOTA; FUNGI; HYDROXY COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; SACCHAROMYCES; STRUCTURAL CHEMICAL ANALYSIS; SYNTHESIS; YEASTS; 550400* - Genetics; 550200 - Biochemistry

Citation Formats

Kumagai, M H, Sverlow, G G, della-Cioppa, G, and Grill, L K. Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter. United States: N. p., 1993. Web. doi:10.1038/nbt0593-606.
Kumagai, M H, Sverlow, G G, della-Cioppa, G, & Grill, L K. Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter. United States. https://doi.org/10.1038/nbt0593-606
Kumagai, M H, Sverlow, G G, della-Cioppa, G, and Grill, L K. 1993. "Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter". United States. https://doi.org/10.1038/nbt0593-606.
@article{osti_6084916,
title = {Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter},
author = {Kumagai, M H and Sverlow, G G and della-Cioppa, G and Grill, L K},
abstractNote = {A recombinant Saccharomyces cerevisiae, expressing and secreting rice [alpha]-amylase, converts starch to ethanol. The rice [alpha]-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N[sub 3]-GCTTCCAA-N[sub 5]-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha, and Candida biodinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch. 45 refs., 8 figs.},
doi = {10.1038/nbt0593-606},
url = {https://www.osti.gov/biblio/6084916}, journal = {Bio/Technology; (United States)},
issn = {0733-222X},
number = ,
volume = 11:5,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1993},
month = {Sat May 01 00:00:00 EDT 1993}
}