Hepatic prolactin binding is rapidly altered by endotoxin in lactating mice
Endotoxin or lipopolysaccharide (LPS), a component of the outer membrane of gram-negative bacteria, produces profound physiologic changes in most mammals. The effects of LPS on ovine prolactin (oPRL) binding by hepatic membranes of lactating mice is explored in this report. Specific /sup 125/I-oPRL binding by liver membranes from LPS-responder C3HfB/HeN mice increased two-fold within fifteen minutes of the injection of LPS, while no change was observed in the non-responder C3H/HeJ mice. Specific /sup 125/I-insulin binding did not change. Scatchard analysis of equilibrium binding of oPRL to C3HfB/HeN liver membranes indicated that within fifteen minutes of LPS injection, a receptor of differing binding affinity appears and then disappears by one hour post-injection. The authors propose that these rapid alterations in the specific binding of oPRL by liver membranes from LPS-injected, lactating C3HfB/HeN mice are due to the transient creation or unmasking of a novel class of PRL receptor. 32 references, 6 figures.
- Research Organization:
- Louisiana State Univ. Medical Center, New Orleans
- OSTI ID:
- 6080583
- Journal Information:
- Life Sci.; (United States), Vol. 41:12
- Country of Publication:
- United States
- Language:
- English
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ENDOTOXINS
BIOLOGICAL FUNCTIONS
LIPOPOLYSACCHARIDES
LTH
BIOCHEMICAL REACTION KINETICS
RECEPTORS
CELL MEMBRANES
INSULIN
IODINE 125
LACTATION
LIVER
MICE
TRACER TECHNIQUES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
GLANDS
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
TOXIC MATERIALS
TOXINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques