Biotinylated erythrocytes: in vivo survival and in vitro recovery

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Blood; (United States)

OSTI ID:6080486

Suzuki, T; Dale, G L

Rabbit erythrocytes were biotinylated by reaction with N-hydroxysuccinimidobiotin; the average level of biotinylation was 25,000 molecules per erythrocyte. These biotinylated cells exhibited a normal survival rate when reinfused into rabbits. Two studies demonstrated that the biotin label was stable in vivo. The first was a double-labeling experiment where the biotinylated erythrocytes were also labeled with /sup 14/C-cyanate; on reinfusion, the loss of biotinylated erythrocytes and /sup 14/C-cyanate label occurred in unison. The second study demonstrated that biotinylated erythrocytes that had been reinfused into rabbits could later be selectively isolated by attachment to an avidin support. This technique will facilitate a variety of studies that require the ability to label a specific cohort of cells in vitro and then reisolate those same cells after in vivo recirculation.

• Research Organization: Scripps Clinic and Research Foundation, La Jolla, CA
• OSTI ID: 6080486
• Journal Information: Blood; (United States), Vol. 70:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOTIN
BIOLOGICAL FUNCTIONS
ERYTHROCYTES
BIOLOGICAL RECOVERY
DOUBLE LABELLING
SURVIVAL CURVES
CARBON 14 COMPOUNDS
CYANATES
CYTOLOGICAL TECHNIQUES
IN VITRO
IN VIVO
RABBITS
TRACER TECHNIQUES
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
FUNCTIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MATERIALS
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RECOVERY
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques