Tn5 insertion mutants of Pseudomonas aeruginosa deficient in surface expression of ferripyochelin-binding protein

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:6080026

Sokol, P A

Transposon (Tn5) insertion mutants were isolated in Pseudomonas aeruginosa PAO. These mutants were screened for expression of the ferripyochelin-binding protein with monoclonal antibody in a whole-cell immunoblot assay. Fourteen mutants were identified which did not express ferripyochelin-binding protein on the cell surface. These mutants did not take up /sup 59/Fe-labeled pyochelin and grew slowly in the presence of iron chelators.

• Research Organization: Univ. of Calgary Health Sciences Centre, Alberta
• OSTI ID: 6080026
• Journal Information: J. Bacteriol.; (United States), Vol. 169:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IRON COMPOUNDS
BIOCHEMICAL REACTION KINETICS
METALLOPROTEINS
GENETIC MAPPING
CHELATING AGENTS
IMMUNOASSAY
IRON 59
LABELLED COMPOUNDS
MONOCLONAL ANTIBODIES
MUTANTS
TRACER TECHNIQUES
ANTIBODIES
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EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAPPING
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRANSITION ELEMENT COMPOUNDS
550201* - Biochemistry- Tracer Techniques