Human aspartic protease genes homologous to progastricsin gene
Two human genomic libraries in lambda-bacteriophages were probed with human progastricsin cDNA and 30 positive clones were identified and isolated. The restriction mapping of the insert DNA of these clones indicated that they could be divided into at least 3 groups based on structural overlaps. Two of the groups have been partially mapped and its restriction enzyme fragments have been probed with 5'- and 3'-region of human progastricsin cDNA. The first group, which will be referred to as GX-1 gene, contained in about 10 kb genomic DNA and probed positive for the progastricsin 3'- and 5'-regions. A 1.3 kb fragment positive in the Southern blot was subcloned in M13 phage and partially sequenced. This structure revealed a 0.2 kb exon with the inferred amino acid sequence homologous to human gastricsin. Since GX-1 gene is positive for both the 5'-and 3'-region of progastricsin it is probably the full length gene of another human aspartic protease with structure very homologous to progastricsin. The clones from a second gene, GX-2, which is about 16 kb represent mainly the 3'-region of progastricsin. Partial nucleotide sequence of presumed exons in GX-2 indicated that it is also homologous to progastricsin. However, the restriction map of GX-2 was very different from GX-1. They speculated that GX-1 and Gx-2 may represent two other genes with exons closely homologous to human progastricsin but more distantly homologous to pepsinogen.
- Research Organization:
- Univ. of Oklahoma Health Science Center, Oklahoma City
- OSTI ID:
- 6076992
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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