Regulation of. beta. -1,4-endoglucanase synthesis in Thermomonospora fusca

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6076556

Lin, E; Wilson, D B

In Thermomonospora fusca YX, endocellulase synthesis varies over a 100-fold range depending on the carbon source used. This study shows that the variation is caused by two regulator mechanisms: an induction mechanisms that increases the rate of endocellulase synthesis about 20-fold and a growth rate-dependent repression mechanism that changes the rate of synthesis over a 6-fold range in both induced and noninduced cells. In T. fusca, endocellulase synthesis can be induced by cellulose, cellobiose, or cellodextrin. Cellulase is involved in inducer generation from cellulose. Growth rate-dependent repression can be reversed by limiting cultures for carbon, nitrogen, or, to a lesser extent, phosphorus. Further evidence for two separate regulatory mechanisms is provided by the isolation of mutants (CC-1 and CC-2) whose endocellulases are synthesized constitutively but are still sensitive to growth rate-dependent repression. These conclusions about total endocellulase synthesis were extended to the individual endocellulases by showing that three T. fusca endocellulases are coordinately regulated.

• Research Organization: Cornell Univ., Ithaca, NY
• OSTI ID: 6076556
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 53:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCES
ENZYME ACTIVITY
CELLULASE
ENZYME INDUCTION
BIOLOGICAL PATHWAYS
CELLOBIOSE
CELLULOLYTIC ACTIVITY
CELLULOSE
MUTANTS
VARIATIONS
BACTERIA
CARBOHYDRATES
DISACCHARIDES
ENZYMES
GENE REGULATION
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
550200* - Biochemistry
550700 - Microbiology