Binding of acyl CoA by fatty acid binding protein and the effect on fatty acid activation
The ability of purified rat liver and heart fatty acid binding proteins (FABPs) to bind oleoyl CoA and modulate acyl CoA synthesis by microsomal membranes was investigated. Using binding assays employing either Lipidex 1000 or multilamellar liposomes to sequester unbound ligand, rat liver but not rat heart FABP was shown to bind radiolabeled acyl CoA. Binding studies suggest that liver FABP has a single binding site for acyl CoA which is separate from the two binding sites for fatty acids. Experiments were then performed to determine how binding may influence acyl CoA metabolism by liver microsomes or heart sarcoplasmic reticulum. Using liposomes as fatty acid donors, liver FABP stimulated acyl CoA production whereas heart FABP did not stimulate production over control values. /sup 14/C-Fatty acid-FABP complexes were prepared, incubated with membranes and acyl CoA synthetase activity was determined. Up to 70% of the fatty acid could be converted to acyl CoA in the presence of liver FABP but in the presence of heart FABP, only 45% of the fatty acid was converted. The amount of product formed was not changed by additional membrane, enzyme cofactor, or incubation time. Liver but not heart FABP bound the acyl CoA formed and removed it from the membranes. These studies suggest that liver FABP can increase the amount of acyl CoA by binding this ligand thereby removing it from the membrane and possibly aiding transport within the cell.
- Research Organization:
- Boston Univ. School of Medicine, MA
- OSTI ID:
- 6074642
- Report Number(s):
- CONF-870644-; TRN: 87-037134
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
METABOLISM
COENZYMES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
LIGASES
ENZYME ACTIVITY
PROTEINS
RECEPTORS
CARBON 14 COMPOUNDS
CELL MEMBRANES
HEART
LIGANDS
LIPOSOMES
LIVER
MICROSOMES
RATS
TRACER TECHNIQUES
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
REACTION KINETICS
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques