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Title: Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells

Abstract

An automatic procedure for recovering the DNA content distribution of mouse irradiated testis cells from flow cytometric histograms is presented. First, a suitable mathematical model is developed, to represent the pattern of DNA content and fluorescence distribution in the sample. Then a parameter estimation procedure, based on the maximum likelihood approach, is constructed by means of an optimization technique. This procedure has been applied to a set of DNA histograms relative to different doses of 0.4-MeV neutrons and to different time intervals after irradiation. In each case, a good agreement between the measured histograms and the corresponding fits has been obtained. The results indicate that the proposed method for the quantitative analysis of germ cell DNA histograms can be usefully applied to the study of the cytotoxic and mutagenic action of agents of toxicological interest such as ionizing radiations.18 references.

Authors:
; ; ;
Publication Date:
Research Org.:
Istituto di Analisi dei Sistemi ed Informatica del CNR, Rome (Italy)
OSTI Identifier:
6073001
Resource Type:
Journal Article
Journal Name:
Cytometry (Baltimore); (United States)
Additional Journal Information:
Journal Volume: 10:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; QUANTITATIVE CHEMICAL ANALYSIS; SPERMATOZOA; GENETIC RADIATION EFFECTS; CELL FLOW SYSTEMS; FLUORESCENCE; IONIZING RADIATIONS; MATHEMATICAL MODELS; MICE; NEUTRONS; REVIEWS; TESTES; ANIMALS; BARYONS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BODY; CHEMICAL ANALYSIS; DOCUMENT TYPES; ELEMENTARY PARTICLES; FERMIONS; GAMETES; GENETIC EFFECTS; GERM CELLS; GONADS; HADRONS; LUMINESCENCE; MALE GENITALS; MAMMALS; NUCLEIC ACIDS; NUCLEONS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; RODENTS; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Lampariello, F, Mauro, F, Uccelli, R, and Spano, M. Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells. United States: N. p., 1989. Web. doi:10.1002/cyto.990100111.
Lampariello, F, Mauro, F, Uccelli, R, & Spano, M. Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells. United States. https://doi.org/10.1002/cyto.990100111
Lampariello, F, Mauro, F, Uccelli, R, and Spano, M. 1989. "Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells". United States. https://doi.org/10.1002/cyto.990100111.
@article{osti_6073001,
title = {Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells},
author = {Lampariello, F and Mauro, F and Uccelli, R and Spano, M},
abstractNote = {An automatic procedure for recovering the DNA content distribution of mouse irradiated testis cells from flow cytometric histograms is presented. First, a suitable mathematical model is developed, to represent the pattern of DNA content and fluorescence distribution in the sample. Then a parameter estimation procedure, based on the maximum likelihood approach, is constructed by means of an optimization technique. This procedure has been applied to a set of DNA histograms relative to different doses of 0.4-MeV neutrons and to different time intervals after irradiation. In each case, a good agreement between the measured histograms and the corresponding fits has been obtained. The results indicate that the proposed method for the quantitative analysis of germ cell DNA histograms can be usefully applied to the study of the cytotoxic and mutagenic action of agents of toxicological interest such as ionizing radiations.18 references.},
doi = {10.1002/cyto.990100111},
url = {https://www.osti.gov/biblio/6073001}, journal = {Cytometry (Baltimore); (United States)},
number = ,
volume = 10:1,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}