Light microscopic autoradiography for study of early changes in the distribution of water-soluble materials

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Journal of Histochemistry and Cytochemistry; (USA)

DOI:https://doi.org/10.1177/38.12.2254645· OSTI ID:6072469

Kawamoto, T ^[1]

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1. Tsurumi Univ., Yokohama (Japan)

An approach using autoradiography for the study of early changes in the distribution of water-soluble materials and the chemography involved was investigated. Radioactive calcium chloride (45Ca) was injected into the iliac vein of a rat. Ten seconds after the injection the rat was frozen in hexane (-90 degrees C). The frozen rat was embedded in 5% sodium carboxymethyl cellulose and blocked in the coolant. A sheet of plastic tape coated with a synthetic rubber glue was fastened to the trimmed block surface, and whole-body sections 2-10 microns thick were cut with a disposable microtome knife. Selected sections were freeze-dried and then covered with a dried autoradiographic emulsion film about 1 microns thick. The autoradiograph clearly showed the distribution of radioactive calcium in the calcification zone of long bones. The samples chosen to assess chemographic artifacts showed positive and negative chemographies on most of the tissues when these were kept at 23 degrees C, and although both chemographic effects were significantly reduced when the samples were kept at -20 degrees C, cells in several tissues still exhibited positive and negative chemographies. The technique can be used for the study of any animal whose size is suitable for whole-body freeze-sectioning.

• OSTI ID: 6072469
• Journal Information: Journal of Histochemistry and Cytochemistry; (USA), Vol. 38:12; ISSN 0022-1554
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
TISSUE DISTRIBUTION
VEINS
AUTORADIOGRAPHY
CALCIUM 45
HISTOLOGICAL TECHNIQUES
MICROSCOPY
RATS
SAMPLE PREPARATION
SKELETON
SOLUBILITY
WATER
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELEMENTS
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
METALS
NUCLEI
ORGANS
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques