Solid-phase receptor binding assay for /sup 125/I-hCG
A solid-phase radioligand-receptor assay (RRA) to measure the binding of /sup 125/I-labelled human chorionic gonadotropin (/sup 125/I-hCG) to target cell membranes has been developed. The binding of /sup 125/I-hCG to membranes immobilized on the wells of microtitration plates reached a maximum at about 3 hours at 37 degrees C, was saturable, displayed a high affinity (Ka = 2.4 X 10(9) M-1) and was specifically inhibited by unlabelled hCG. In comparison with RRAs carried out with membranes in suspension, the solid-phase RRA is significantly simpler and much faster to perform as it avoids centrifugation or filtration procedures. The solid-phase RRA was adapted profitably to process large numbers of samples at the same time. It proved particularly useful as a screening assay to detect anti-hCG monoclonal antibodies with high inhibitory activity for binding of /sup 125/I-hCG to its receptors.
- Research Organization:
- Univ. of Padova, Italy
- OSTI ID:
- 6072260
- Journal Information:
- J. Immunoassay; (United States), Vol. 8:2-3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GONADOTROPINS
BIOCHEMICAL REACTION KINETICS
RADIOASSAY
RECEPTORS
CELL MEMBRANES
IODINE 125
LIGANDS
MONOCLONAL ANTIBODIES
SWINE
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VERTEBRATES
550301* - Cytology- Tracer Techniques