/sup 1/H and /sup 31/P NMR spectra of rat sarcoma
/sup 1/H and /sup 31/P spectra of the BA 1112 sarcoma of live rats were recorded in a 2 Tesla GE CSI spectrometer with the 2 cm /sup 1/H and /sup 31/P surface coils. Water was suppressed by the 1331 pulse sequence. In the aliphatic region of the /sup 1/H spectrum a major resonance was observed corresponding to the -(CH/sub 2/)/sub n/- protons of fatty acyl chains. Several minor resonances from various -CH/sub 2/- protons of fatty acyl chains were also present. In addition they have identified the -N(CH/sub 3/)/sub 3/ group of choline. In the aromatic region of the /sup 1/H spectrum the major resonance at 7.92 ppm was of unknown nature. Minor resonances from protons of the histidine ring in anserine and adenine nucleotides could be easily discerned. The /sup 31/P spectrum of the sarcoma contained only P/sub i/ as a significant resonance, with a chemical shift corresponding to a pH of 6.5. Their /sup 1/H spectra confirm the observation of Mountford et al. that the fatty acyl chains of membrane lipids are mobile in cancer cells. Since their spectra were obtained on live animals, their data have the potential for tumor diagnosis by /sup 1/H NMR. Their /sup 31/P spectra are in agreement with the general view that glycolysis is the predominant metabolism in tumors resulting in an acidic pH.
- Research Organization:
- Univ. of Illinois, Chicago
- OSTI ID:
- 6071635
- Report Number(s):
- CONF-870644-; TRN: 87-037431
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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SARCOMAS
MOLECULAR STRUCTURE
NMR SPECTRA
CHEMICAL SHIFT
CHOLINE
LIPIDS
NUCLEAR MAGNETIC RESONANCE
PH VALUE
PHOSPHORUS 31
PROTONS
RATS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BARYONS
DISEASES
DRUGS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MAMMALS
NEOPLASMS
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques