Relationship between RNA polymerase II and efficiency of vaccinia virus replication
It is clear from previous studies that host transcriptase or RNA polymerase II (pol II) has a role in poxvirus replication. To elucidate the participation of this enzyme further, in this study the authors examined several parameters related to pol II during the cycle of vaccinia virus infection in L-strain fibroblasts, HeLa cells, and L/sub 6/H/sub 9/ rat myoblasts. Nucleocytoplasmic transposition of pol II into virus factories and virions was assessed by immunofluorescence and immunoblotting by using anti-pol II immunoglobulin G. RNA polymerase activities were compared in nuclear extracts containing cured enzyme preparations. Rates of translation into cellular or viral polypeptides were ascertained by labeling with (/sup 35/S)methionine. In L and HeLa cells, which produced vaccinia virus more abundantly, the rate of RNA polymerase and translation in controls and following infection were higher than in myoblasts. The data on synthesis and virus formation could be correlated with observations on transmigration of pol II, which was more efficient and complete in L and HeLa cells. The stimulus for pol II to leave the nucleus required the expression of both early and late viral functions. On the basis of current and past information, the authors suggest that mobilization of pol II depends on the efficiency of vaccinia virus replication and furthermore that control over vaccinia virus production by the host is related to the content or availability (or both) of pol II in different cell types.
- Research Organization:
- Univ. of Western Ontario, London (Canada)
- OSTI ID:
- 6070181
- Journal Information:
- J. Virol.; (United States), Vol. 63:4
- Country of Publication:
- United States
- Language:
- English
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RNA POLYMERASES
BIOCHEMICAL REACTION KINETICS
VIRUSES
INFECTIVITY
ANIMAL CELLS
CELL NUCLEI
CYTOPLASM
EFFICIENCY
ENZYME ACTIVITY
FIBROBLASTS
FLUORESCENCE
HELA CELLS
IMMUNOGLOBULINS
METHIONINE
PATHOGENESIS
RATS
SULFUR 35
TRACER TECHNIQUES
VIRAL DISEASES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GLOBULINS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
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PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
SULFUR ISOTOPES
TRANSFERASES
VERTEBRATES
550901* - Pathology- Tracer Techniques