Effects of SO/sub 2/ exposure on canine pulmonary epithelial functions

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148909087852· OSTI ID:6066650

Man, S F; Hulbert, W C; Man, G; Mok, K; Williams, D J

We examined the effects of a single exposure of high concentrations of sulfur dioxide (SO/sub 2/) on the pulmonary epithelium in adult dogs over a period of several weeks. Mucociliary tracheal transport rates and alveolar clearance of /sup 99m/Tc-labeled diethylene triamine pentacetate (/sup 99m/TcO/sub 4/minus//) were measured in vivo, before and immediately after inhalation of 100 ppm or 500 ppm SO/sub 2/, and then weekly for 3-5 weeks. At the completion of the in vivo studies, tracheal epithelium was studied in Ussing chambers for bioelectric properties (short-circuited current, transepithelial potential difference), nonelectrolyte permeability for calculation of pore sizes, and changes in bioelectric properties following pharmacological manipulations. These tissues were then fixed for scanning electron microscopy studies. Additional dogs were sacrificed for microscopy studies at several time intervals to provide a histological basis for the altered mucociliary transport. We found that despite marked derangement of mucociliary transport caused by damage to the ciliated cells, recovery occurred over a period of several weeks, and alveolar permeability as assessed by the radioaerosol technique did not change. We concluded that the solubility of SO/sub 2/ and perhaps a more severe damaging effect of SO/sub 2/ specific on the ciliated cells might be the explanation for the observations.

• Research Organization: Univ. of Alberta, Edmonton (Canada)
• OSTI ID: 6066650
• Journal Information: Exp. Lung Res.; (United States), Vol. 15:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LUNGS
SENSITIVITY
SULFUR DIOXIDE
TOXICITY
ACUTE EXPOSURE
AEROSOLS
DOGS
DTPA
EPITHELIUM
INHALATION
ISOMERIC NUCLEI
LUNG CLEARANCE
METABOLISM
SCANNING ELECTRON MICROSCOPY
TECHNETIUM 99
TRACER TECHNIQUES
TRACHEA
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHALCOGENIDES
CHELATING AGENTS
CLEARANCE
COLLOIDS
DISPERSIONS
DRUGS
ELECTRON MICROSCOPY
EXCRETION
HOURS LIVING RADIOISOTOPES
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
SOLS
SULFUR COMPOUNDS
SULFUR OXIDES
TECHNETIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques