Distribution and HPLC study of chromium-51 binding sites in Chinese Hamster ovary cells

Journal Article · Thu Jan 01 00:00:00 EST 1987 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398709531026· OSTI ID:6064664

Denniston, M L; Uyeki, E M

Chinese hamster ovary cells were cultured with chromium-51 chromate to study the site of chromium interaction with cell biomolecules. After incubation, cells were homogenized and separated into nuclear, mitochondrial, microsomal, and cytosolic fractions. Greater than 75% of the radioactivity was found in the cytosolic fraction. The supernatant from the centrifuged cell homogenate, which contained > 90% of the chromium radioactivity, was subjected to chromatographic investigation. The combination of anion exchange and ion-pair high-performance liquid chromatography (HPLC) showed that at least three different molecular species interact with chromate or its reduced derivative, Cr(III). These species are glutathione, the nucleotides cytosine triphosphate, uridine triphosphate, guanine triphosphate, adenosine triphosphate, and adenosine diphosphate, plus an as yet unknown species of protein or peptide. Preliminary data for the specific activity of nucleoside triphosphates range from 6000 to 18,000 cpm/..mu..g ribonucleoside triphosphate. The glutathione accounted for 50% of the observed radioactivity, the nucleotides for 30%, and the metalloprotein accounted for the remainder.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 6064664
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 21:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CHROMIUM 51
TISSUE DISTRIBUTION
CHROMIUM COMPOUNDS
STRUCTURE-ACTIVITY RELATIONSHIPS
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CHEMICAL STATE
CHO CELLS
GLUTATHIONE
METALLOPROTEINS
NUCLEOTIDES
RADIOACTIVITY
TOXICITY
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
CHROMIUM ISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
TRANSITION ELEMENT COMPOUNDS
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques