Comparison of the indirect immunobead, radiolabeled, and immunofluorescence assays for immunoglobulin G serum antibodies to human sperm

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Fertility and Sterility; (USA)

OSTI ID:6062336

Haas, Jr, G G; D'Cruz, O J; DeBault, L E ^[1]

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1. Univ. of Oklahoma Health Sciences Center, Oklahoma City (USA)

The relative sensitivities of the indirect immunobead test, the indirect flo cytometric immunofluorescence assay, and an indirect radiolabeled antiglobulin assay were compared. Eighteen immunobead test positive sera and 18 negative sera were used as the standard for the other two assays. Of the 18 positive sera, 14 (77%) and 5 (27%) were positive in the immunofluorescence assay and the radiolabeled antiglobulin assay, respectively. Four (22%) of the low titer immunobead test positive sera were negative by both the immunofluorescence assay and the radiolabeled antiglobulin assay. However, there was a significant positive correlation between the results of the immunofluorescence assay and the radiolabeled antiglobulin assay (r = 0.73) and between the results of the radiolabeled antiglobulin assay and the titer of the immunobead test (r = 0.82). The use of an unselected sperm population in the radiolabeled antiglobulin assay and the classical indirect immunofluorescence method using methanol-fixed sperm gave false-positive results in the radiolabeled antiglobulin assay and the immunofluorescence assay. These results suggested that immunoglobulin G antisperm antibody positive sera may be reactive both to sperm surface and internalized sperm antigens.

• OSTI ID: 6062336
• Journal Information: Fertility and Sterility; (USA), Vol. 55:2; ISSN 0015-0282
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNOGLOBULINS
IMMUNOASSAY
SPERMATOZOA
ANTIBODIES
COMPARATIVE EVALUATIONS
FLUORESCENCE
IODINE ISOTOPES
RADIOIMMUNOASSAY
BIOASSAY
DIAGNOSTIC TECHNIQUES
GAMETES
GERM CELLS
GLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques