Localization and quantitation of tritiated compounds in tissue sections with a gaseous detector of beta particles: Comparison with film autoradiography

Journal Article · Fri Feb 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.88.4.1466· OSTI ID:6062007

Tribollet, E; Dreifuss, J J; Charpak, G; Dominik, W; Zaganidis, N ^[1]

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1. Univ. Medical Centre, Geneva (Switzerland)

Quantitative analysis of tritium polymer standards and of brain sections labeled with tritiated vasopressin was carried out by using a gaseous detector of beta particles designed for this purpose. The gaseous detector showed major advantages compared with film autoradiography: the linearity and the large dynamic range of intensity measurements as well as the short time needed for data acquisition.

• OSTI ID: 6062007
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 88:4; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
COMPARATIVE EVALUATIONS
RADIATION DETECTORS
VASOPRESSIN
BIOLOGICAL LOCALIZATION
BRAIN
DATA ACQUISITION
QUANTITATIVE CHEMICAL ANALYSIS
RATS
TRITIUM COMPOUNDS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
HORMONES
HYDROGEN COMPOUNDS
MAMMALS
MEASURING INSTRUMENTS
NERVOUS SYSTEM
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques