Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain

Borek, J P; Guilarte, T R

Title: Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain

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Abstract

A dietary deficiency of vitamin B-6 promotes seizure activity in neonatal animals and human infants. Previous studied have shown that neonatal vitamin B-6 deprivation results in reduced levels of brain gamma-aminobutyric acid (GABA) and increased binding at the GABA site of the GABA/BDZ receptor complex. Since the GABA and BDZ receptors are allosterically linked, this study was undertaken to determine if vitamin B-6 deprivation had an effect on BDZ receptor binding. Benzodiazepine receptor binding isotherms using {sup 3}H-flunitrazepam as ligand were performed in the presence and absence of 10 {mu}M GABA. The results indicate a significant increase in the binding affinity (Kd) in the presence of GABA in cerebellar membranes from deficient rat pups at 14 days of age with no effect on receptor number (Bmax). By 28 days of age, the increase in Kd was no longer present. No change in Kd or Bmax was observed in cortical tissue from deficient animals at 14 or 28 days of age. Preliminary studies of GABA-enhancement of {sup 3}H-flunitrazepam binding indicate that vitamin B-6 deficiency also induces alterations in the ability of GABA to enhance BZD receptor binding. In summary, these results indicate that the effects of vitamin B-6 deprivation on BDZmore »« less

Authors:

Borek, J P; Guilarte, T R ^[1]

Johns Hopkins Univ., Baltimore, MD (United States)

Publication Date:: Mon Feb 26 00:00:00 EST 1990

OSTI Identifier:: 6061947

Report Number(s):: CONF-9104107-
Journal ID: ISSN 0892-6638; CODEN: FAJOE

Resource Type:: Conference

Journal Name:: FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

Additional Journal Information:: Journal Volume: 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; Journal ID: ISSN 0892-6638

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; HYPNOTICS AND SEDATIVES; BIOCHEMICAL REACTION KINETICS; PYRIDOXINE; BIOLOGICAL EFFECTS; AFFINITY; AGE DEPENDENCE; AMINOBUTYRIC ACID; BRAIN; NEONATES; NUTRITIONAL DEFICIENCY; RATS; RECEPTORS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZINES; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; PYRIDINES; REACTION KINETICS; RODENTS; VERTEBRATES; VITAMIN B GROUP; VITAMINS; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    Borek, J P, and Guilarte, T R. Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain.  United States: N. p., 1990. 
        Web.

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                    Borek, J P, & Guilarte, T R. Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain.  United States.

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                    Borek, J P, and Guilarte, T R. 1990.  
        "Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain".  United States.

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@article{osti_6061947,

  title        = {Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain},

  author       = {Borek, J P and Guilarte, T R},

  abstractNote = {A dietary deficiency of vitamin B-6 promotes seizure activity in neonatal animals and human infants. Previous studied have shown that neonatal vitamin B-6 deprivation results in reduced levels of brain gamma-aminobutyric acid (GABA) and increased binding at the GABA site of the GABA/BDZ receptor complex. Since the GABA and BDZ receptors are allosterically linked, this study was undertaken to determine if vitamin B-6 deprivation had an effect on BDZ receptor binding. Benzodiazepine receptor binding isotherms using {sup 3}H-flunitrazepam as ligand were performed in the presence and absence of 10 {mu}M GABA. The results indicate a significant increase in the binding affinity (Kd) in the presence of GABA in cerebellar membranes from deficient rat pups at 14 days of age with no effect on receptor number (Bmax). By 28 days of age, the increase in Kd was no longer present. No change in Kd or Bmax was observed in cortical tissue from deficient animals at 14 or 28 days of age. Preliminary studies of GABA-enhancement of {sup 3}H-flunitrazepam binding indicate that vitamin B-6 deficiency also induces alterations in the ability of GABA to enhance BZD receptor binding. In summary, these results indicate that the effects of vitamin B-6 deprivation on BDZ receptor binding are region specific and age related.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6061947},
  journal      = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},

  issn         = {0892-6638},

  number       = ,

  volume       = 4:3,

  place        = {United States},

  year         = {Mon Feb 26 00:00:00 EST 1990},

  month        = {Mon Feb 26 00:00:00 EST 1990}

}

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