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Title: Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody

Abstract

Class-switched monoclonal antibody SV2-61r recognized the extracellular domain of c-erbB-2 protooncogene products separate from the epidermal growth factor receptor. We studied the potential of SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells, which has been reported to have prognostic value in adenocarcinoma patients. Radiolabeled SV2-61r specifically bound to various adenocarcinoma cells in addition to c-erbB-2-transfected NIH-3T3 cells (A4) with the affinity constant of 4.4 x 10(8) M-1. SV2-61r injected i.v. localized well to A4 cells xenografted in nude mice. Tumor uptake and localization index of radioiodinated SV2-61r were lower than those of 111In-labeled SV2-61r, probably due to the internalization and dehalogenation of formed antibody-antigen complexes. Biodistribution and specificity of targeting were assessed by comparison among three cells, A4, lung cancer SBC-3 (c-erbB-2 weakly positive) and B-lymphoblastoid Manca cells (c-erbB-2 negative). Tumor:blood ratios, obtained 48 h after injection, were 5.63, 1.45, and 0.68, respectively, indicating the potential of 111In-labeled SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells. Because of its close relationship with carcinogenesis and the uniform expression, c-erbB-2 protooncogene products seem to be the optimal target of imaging and therapy of adenocarcinoma patients.

Authors:
; ; ; ; ; ; ; ; ;  [1]
  1. Kyoto Univ. School of Medicine (Japan)
Publication Date:
OSTI Identifier:
6060124
Resource Type:
Journal Article
Journal Name:
Cancer Research; (USA)
Additional Journal Information:
Journal Volume: 51:3; Journal ID: ISSN 0008-5472
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOMAS; DIAGNOSIS; GROWTH FACTORS; RECEPTORS; EPIDERMIS; EXPERIMENTAL NEOPLASMS; INDIUM 111; MICE; MONOCLONAL ANTIBODIES; ONCOGENES; RADIOIMMUNOSCINTIGRAPHY; TUMOR CELLS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BODY; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EPITHELIUM; GENES; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; MINUTES LIVING RADIOISOTOPES; MITOGENS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOIMMUNODETECTION; RADIOISOTOPE SCANNING; RADIOISOTOPES; RODENTS; SCINTISCANNING; SKIN; TISSUES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Saga, T, Endo, K, Akiyama, T, Sakahara, H, Koizumi, M, Watanabe, Y, Nakai, T, Hosono, M, Yamamoto, T, and Toyoshima, K. Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody. United States: N. p., 1991. Web.
Saga, T, Endo, K, Akiyama, T, Sakahara, H, Koizumi, M, Watanabe, Y, Nakai, T, Hosono, M, Yamamoto, T, & Toyoshima, K. Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody. United States.
Saga, T, Endo, K, Akiyama, T, Sakahara, H, Koizumi, M, Watanabe, Y, Nakai, T, Hosono, M, Yamamoto, T, and Toyoshima, K. 1991. "Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody". United States.
@article{osti_6060124,
title = {Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody},
author = {Saga, T and Endo, K and Akiyama, T and Sakahara, H and Koizumi, M and Watanabe, Y and Nakai, T and Hosono, M and Yamamoto, T and Toyoshima, K},
abstractNote = {Class-switched monoclonal antibody SV2-61r recognized the extracellular domain of c-erbB-2 protooncogene products separate from the epidermal growth factor receptor. We studied the potential of SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells, which has been reported to have prognostic value in adenocarcinoma patients. Radiolabeled SV2-61r specifically bound to various adenocarcinoma cells in addition to c-erbB-2-transfected NIH-3T3 cells (A4) with the affinity constant of 4.4 x 10(8) M-1. SV2-61r injected i.v. localized well to A4 cells xenografted in nude mice. Tumor uptake and localization index of radioiodinated SV2-61r were lower than those of 111In-labeled SV2-61r, probably due to the internalization and dehalogenation of formed antibody-antigen complexes. Biodistribution and specificity of targeting were assessed by comparison among three cells, A4, lung cancer SBC-3 (c-erbB-2 weakly positive) and B-lymphoblastoid Manca cells (c-erbB-2 negative). Tumor:blood ratios, obtained 48 h after injection, were 5.63, 1.45, and 0.68, respectively, indicating the potential of 111In-labeled SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells. Because of its close relationship with carcinogenesis and the uniform expression, c-erbB-2 protooncogene products seem to be the optimal target of imaging and therapy of adenocarcinoma patients.},
doi = {},
url = {https://www.osti.gov/biblio/6060124}, journal = {Cancer Research; (USA)},
issn = {0008-5472},
number = ,
volume = 51:3,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1991},
month = {Fri Feb 01 00:00:00 EST 1991}
}