Production of SCP and cellulase by Aspergillus terreus from bagasse substrate

Garg, S K; Neelakantan, S

doi:10.1002/bit.260241108

Title: Production of SCP and cellulase by Aspergillus terreus from bagasse substrate

Full Record
Other Related Research

Abstract

The fermentation of 1.0% untreated bagasse under optimum cultural and nutritional conditions with Aspergillus terreus GN1 indicated that the maximum rate of protein and cellulase production could be obtained during three days of submerged fermentation. Even though 16.4% protein recovery, 0.55 units CMCase/mL, and 0.027 FPase units/mL were obtained on the seventh day, the rates of increase in protein recovery and cellulase production were slower than those obtained up to three days, which were 14.3% protein recovery, 0.45 units cMCase/mL, and 0.019 units FPase/mL. There was an initial lag in the utilization of cellulose up to two days due to the utilization of the water-soluble carbohydrate present in untreated bagasse. Cellulose utilization and water-soluble carbohydrate content during fermentation were correlated with protein recovery and enzyme production. The protein and cellulase production during three days fermentation with 1.0% untreated and treated bagasse were compared and the protein content of the total biomass was calculated into constituent protein contributed by the fungal mycelium and the undegraded bagasse. The total biomass recovered with untreated and treated bagasse was 1020 and 820 mg/g bagasse substrate, respectively, and contained 14.3 and 20.6% crude protein, respectively. The contribution of fungal biomass and undegraded bagasse was 309more »« less

Authors:: Garg, S K; Neelakantan, S

Publication Date:: Fri Jan 01 00:00:00 EST 1982

Research Org.:: Dairy Microbiology Div, National Dairy Research Inst, Karnal - 132001 (Haryara), India

OSTI Identifier:: 6060000

Resource Type:: Journal Article

Journal Name:: Biotechnol. Bioeng.; (United States)

Additional Journal Information:: Journal Volume: 24

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; BAGASSE; FERMENTATION; ASPERGILLUS; CELLULASE; CELLULOSE; SINGLE CELL PROTEIN; SUBSTRATES; AGRICULTURAL WASTES; BIOCONVERSION; CARBOHYDRATES; ENZYMES; FUNGI; GLYCOSYL HYDROLASES; HYDROLASES; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; ORGANIC WASTES; PLANTS; POLYSACCHARIDES; SACCHARIDES; WASTES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology

Citation Formats


                    Garg, S K, and Neelakantan, S. Production of SCP and cellulase by Aspergillus terreus from bagasse substrate.  United States: N. p., 1982. 
        Web.  doi:10.1002/bit.260241108.

Copy to clipboard


                    Garg, S K, & Neelakantan, S. Production of SCP and cellulase by Aspergillus terreus from bagasse substrate.  United States.  https://doi.org/10.1002/bit.260241108

Copy to clipboard


                    Garg, S K, and Neelakantan, S. 1982.  
        "Production of SCP and cellulase by Aspergillus terreus from bagasse substrate".  United States.  https://doi.org/10.1002/bit.260241108.

Copy to clipboard


                    
@article{osti_6060000,

  title        = {Production of SCP and cellulase by Aspergillus terreus from bagasse substrate},

  author       = {Garg, S K and Neelakantan, S},

  abstractNote = {The fermentation of 1.0% untreated bagasse under optimum cultural and nutritional conditions with Aspergillus terreus GN1 indicated that the maximum rate of protein and cellulase production could be obtained during three days of submerged fermentation. Even though 16.4% protein recovery, 0.55 units CMCase/mL, and 0.027 FPase units/mL were obtained on the seventh day, the rates of increase in protein recovery and cellulase production were slower than those obtained up to three days, which were 14.3% protein recovery, 0.45 units cMCase/mL, and 0.019 units FPase/mL. There was an initial lag in the utilization of cellulose up to two days due to the utilization of the water-soluble carbohydrate present in untreated bagasse. Cellulose utilization and water-soluble carbohydrate content during fermentation were correlated with protein recovery and enzyme production. The protein and cellulase production during three days fermentation with 1.0% untreated and treated bagasse were compared and the protein content of the total biomass was calculated into constituent protein contributed by the fungal mycelium and the undegraded bagasse. The total biomass recovered with untreated and treated bagasse was 1020 and 820 mg/g bagasse substrate, respectively, and contained 14.3 and 20.6% crude protein, respectively. The contribution of fungal biomass and undegraded bagasse was 309 and 711, and 373 and 447 mg/g untreated and treated bagasse substrates, respectively. In an 8-L-flask trial during three days of fermentation, the recovery of SCP and cellulase were 66 g and 32,400 units (Sigma) for treated bagasse and 82 g and 8200 units (Sigma) for untreated bagasse, respectively. (Refs. 18).},

  doi          = {10.1002/bit.260241108},

  url          = {https://www.osti.gov/biblio/6060000},
  journal      = {Biotechnol. Bioeng.; (United States)},
number       = ,

  volume       = 24,

  place        = {United States},

  year         = {Fri Jan 01 00:00:00 EST 1982},

  month        = {Fri Jan 01 00:00:00 EST 1982}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1002/bit.260241108

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Effect of nutritional factors on cellulase enzyme and microbial protein production by aspergillus terreus and its evaluation

Journal Article Garg, S; Neelaksamtan, S - Biotechnol. Bioeng.; (United States)

The biomass yield, cellulolytic activity, and protein recovery using Aspergillus terreus GN1 with alkali-treated sugarcane bagasse was studied using different levels (250-600 mg of N/L of broth) of organic and inorganic nitrogen sources, e.g., cattle urine, urea, cornsteep liquor, ammonium sulfate, ammonium nitrate, ammonium iron sulfate, ammonium chloride, and sodium nitrate. Among different levels of alkali-treated bagasse substrate concentrations (0.5-4.0% w/v) tested, 1.0% substrate yielded the highest crude protein content, protein recovery, and cellulolytic activity. The biomass recovery with 1.0% substrate ranged from 290-380 mg/500 mg bagasse substrate in a 50 mL broth with a nitrogen level of 250-600 mgmore »« less
https://doi.org/10.1002/bit.260240110
Sugar-Based Ethanol Biorefinery: Ethanol, Succinic Acid and By-Product Production

Technical Report Day, Donal

The work conducted in this project is an extension of the developments itemized in DE-FG-36-04GO14236. This program is designed to help the development of a biorefinery based around a raw sugar mill, which in Louisiana is an underutilized asset. Some technical questions were answered regarding the addition of a biomass to ethanol facility to existing sugar mills. The focus of this work is on developing technology to produce ethanol and valuable by-products from bagasse. Three major areas are addressed, feedstock storage, potential by-products and the technology for producing ethanol from dilute ammonia pre-treated bagasse. Sugar mills normally store bagasse inmore »« less
https://doi.org/10.2172/950487

Full Text Available
Thermophilic Gram-Positive Biocatalysts for Biomass Conversion to Ethanol

Technical Report Shanmugam, K; Ingram, L; Maupin-Furlow, J; ...

Production of energy from renewable sources is receiving increased attention due to the finite nature of fossil fuels and the environmental impact associated with the continued large scale use of fossil energy sources. Biomass, a CO2-neutral abundant resource, is an attractive alternate source of energy. Biomass-derived sugars, such as glucose, xylose, and other minor sugars, can be readily fermented to fuel ethanol and commodity chemicals. Extracellular cellulases produced by fungi are commercially developed for depolymerization of cellulose in biomass to glucose for fermentation by appropriate biocatalysts in a simultaneous saccharification and fermentation (SSF) process. Due to the differences in themore »« less
https://doi.org/10.2172/882538

Full Text Available
Integrated production of ethanol fuel and protein from coastal bermudagrass

Journal Article De La Rosa, L; Reshamwalka, S; Latimer, V; ...

The herbaceous crops that may provide fermentable carbohydrates for production of fuels and chemicals also contain 10-20% protein. Protein coproduction with biomass-derived fuels and chemicals has important advantages: (1) food and fuel production can be integrated, and (2) protein is a high-value product that may significantly improve overall process economics. The authors report the results of an integrated approach to producing protein and fermentable sugars from one herbaceous species, Coastal Bermudagrass (CBG). The ammonia fiber explosion (AFEX) process makes possible over 90% conversion of cellulose and hemicellulose to simple sugars (about 650 mg reducing sugars/g dry CBG) at 5 IUmore »« less
https://doi.org/10.1007/BF02941823
Ethanol production via fungal decomposition and fermentation of biomass. Semiannual progress report, October 1981-March 1982

Technical Report Antonopoulos, A; Wene, E

During the first half of FY 1982, a few additional Fusarium isolates were obtained and screened for their cellulolytic and fermenting abilities. Experiments with an isolate from Fusarium strain ANL 22-760 showed that cellulose was the best inducer of FPase and CMCase, while glucose, lactose, and xylose almost completely inhibited cellulase production. Tests with Fusarium strain ANL 11182 indicated that a cellulase production of 2.5 IU/mL in 14 days was obtained at 28/sup 0/C in a medium of 1% cellulose and in the presence of 0.7 mg/mL extracellular protein. Increasing the nitrogen content of the medium stimulated cell growth butmore »« less

Similar Records