Effects of in vitro UVA irradiation and PUVA treatment on membrane fatty acids and activities of antioxidant enzymes in human keratinocytes

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Journal of Investigative Dermatology; (USA)

DOI:https://doi.org/10.1111/1523-1747.ep12462271· OSTI ID:6058456

Jansen, C T; Puntala, A; Ahotupa, M ^[1]

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1. Univ. of Turku (Finland)

Human Keratinocytes (NCTC 2544) in culture were exposed to either plain ultraviolet A (UVA) irradiation or to 8-methoxypsoralen plus UVA (PUVA) treatment. Lipid peroxidation, activities of antioxidant enzymes, and percentage amounts of 14C-arachidonic acid in various cellular lipid subclasses and in the culture medium were measured. Both UVA irradiation and PUVA treatment induced significant changes in the distribution of arachidonic acid and increased the liberation of arachidonic acid from membrane phospholipids. At 24 h after either UVA irradiation or PUVA treatment the formation of thiobarbituric acid reactive material was significantly increased, whereas the amount of conjugated dienes was unaffected. The activities of the antioxidant enzymes, catalase and superoxide dismutase, were already significantly decreased at 0.5 h after UVA irradiation or PUVA treatment. The enzyme activities were partially restored during the following 24 h incubation. From the present study, we suggest that in keratinocytes both plain UVA irradiation and PUVA treatment induce changes in the distribution of membrane fatty acids and cause an impairment in the enzymic defense system against oxidative stress.

• OSTI ID: 6058456
• Journal Information: Journal of Investigative Dermatology; (USA), Vol. 96:2; ISSN 0022-202X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
ARACHIDONIC ACID
SUBCELLULAR DISTRIBUTION
CATALASE
ENZYME ACTIVITY
SUPEROXIDE DISMUTASE
CARBON 14 COMPOUNDS
CELL MEMBRANES
EPIDERMIS
MAN
METABOLISM
PHOSPHOLIPIDS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ENZYMES
EPITHELIUM
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PRIMATES
RADIATION EFFECTS
RADIATIONS
SKIN
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
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550201 - Biochemistry- Tracer Techniques