The effects of inferior olive lesion on strychnine seizure

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Brain Research Bulletin; (USA)

DOI:https://doi.org/10.1016/0361-9230(90)90118-J· OSTI ID:6057300

Anderson, M C; Chung, E Y; Van Woert, M H ^[1]

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1. Mount Sinai School of Medicine, New York, NY (USA)

Bilateral inferior olive lesions, produced by systemic administration of the neurotoxin 3-acetylpyridine (3AP) produce a proconvulsant state specific for strychnine-induced seizures and myoclonus. We have proposed that these phenomena are mediated through increased excitation of cerebellar Purkinje cells, through activation of glutamate receptors, in response to climbing fiber deafferentation. An increase in quisqualic acid (QA)-displaceable ({sup 3}H)AMPA ((RS)-alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid) binding in cerebella from inferior olive-lesioned rats was observed, but no difference in ({sup 3}H)AMPA binding displaced by glutamate, kainic acid (KA) or glutamate diethylester (GDEE) was seen. The excitatory amino acid antagonists GDEE and MK-801 ((+)-5-methyl-10,11-dihydro-5H-dibenzo(a,d)cyclo-hepten-5,10 imine) were tested as anticonvulsants for strychnine-induced seizures in 3AP inferior olive-lesioned and control rats. Neither drug effected seizures in control rats, however, both GDEE and MK-801 produced a leftward shift in the strychnine-seizure dose-response curve in 3AP inferior olive-lesioned rats. GDEE also inhibited strychnine-induced myoclonus in the lesioned group, while MK-801 had no effect on myoclonus. The decreased threshold for strychnine-induced seizures and myoclonus in the 3AP-inferior olive-lesioned rats may be due to an increase in glutamate receptors as suggested by the ({sup 3}H)AMPA binding data.

• OSTI ID: 6057300
• Journal Information: Brain Research Bulletin; (USA), Vol. 25:4; ISSN 0361-9230
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTICONVULSANTS
BIOLOGICAL EFFECTS
BRAIN
PATHOLOGICAL CHANGES
GLUTAMIC ACID
RECEPTORS
STRYCHNINE
TOXICITY
BIOCHEMICAL REACTION KINETICS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALOIDS
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
AZOLES
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques