Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus uv-specific endonucleases

Journal Article · Thu Dec 25 00:00:00 EST 1980 · J. Biol. Chem.; (United States)

OSTI ID:6055809

Gordon, L K; Haseltine, W A

A comparison was made of the activity of the uv-specific endonucleases of bacteriophage T4 (T4 endonuclease V) and of Micrococcus luteus on ultraviolet light-irradiated DNA substrates of defined sequence. The two enzyms cleave DNA at the site of pyrimidine dimers with the same frequency. The products of the cleavage reaction are the same. The pyrimidine dimer DNA-glycosylase activity of both enzymes is more active on double-stranded DNA than it is on single-stranded DNA.

• OSTI ID: 6055809
• Journal Information: J. Biol. Chem.; (United States), Vol. 255:24
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DIMERS
RADIOINDUCTION
DNA
BIOLOGICAL RADIATION EFFECTS
ENZYME ACTIVITY
BIOCHEMISTRY
NUCLEASES
BACTERIOPHAGES
MICROCOCCUS LUTEUS
PYRIMIDINES
ULTRAVIOLET RADIATION
AZINES
BACTERIA
BIOLOGICAL EFFECTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550201 - Biochemistry- Tracer Techniques