Identification and characterization of cellular binding proteins (receptors) for recombinant human bone morphogenetic protein 2B, an initiator of bone differentiation cascade

Journal Article · Mon Apr 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.8.3397· OSTI ID:6055802

Paralkar, V M; Reddi, A H ^[1]; Hammonds, R G ^[2]

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1. National Inst. of Health, Bethesda, MD (United States)
2. Genentech, Inc., San Francisco, CA (United States)

Bone morphogenetic protein 2B (BMP 2B), is a heparin-binding bone differentiation factor that initiates endochondral bone formation in rats when implanted subcutaneously. The molecular mechanism of action of this differentiation factor is not known, and as a first step the authors have examined BMP 2B-responsive cells for the presence of specific cellular binding proteins. Using {sup 125}I-labeled BMP 2B, specific high-affinity binding sites for recombinant human BMP 2B on MC3T3 E1 osteoblast-like cells as well as on NIH 3T3 fibroblasts were identified. Platelet-derived growth factor, epidermal growth factor, and transforming growth factor {beta} did not compete for the binding of radiolabeled BMP 2B. The binding of BMP 2B is a time- and temperature-dependent process. Chemical crosslinking of radiolabeled BMP showed two components. These data demonstrate specific, high-affinity cell-surface binding proteins for BMP 2B.

• OSTI ID: 6055802
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:8; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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BONE TISSUES
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CELL DIFFERENTIATION
ELECTROPHORESIS
IODINATION
IODINE 125
MEMBRANE PROTEINS
RATS
RECEPTORS
ANIMAL TISSUES
ANIMALS
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DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
INTERMEDIATE MASS NUCLEI
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IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
TISSUES
TRACER TECHNIQUES
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