Rapid diagnosis of occult abscesses using sup 99m Tc-labeled monoclonal antibodies

Conference · Tue Jan 01 00:00:00 EST 1991

OSTI ID:6055279

Coons, T A; Rhodes, B A ^[1]; Thakur, M L ^[2]; Marcus, C S ^[3]; Ballou, B ^[4]

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1. RhoMed, Inc., Albuquerque, NM (USA)
2. Thomas Jefferson Univ., Philadelphia, PA (USA)
3. Harbor-UCLA Medical Center, Torrance, CA (USA)
4. Pittsburgh Univ., PA (USA)

Acute infections, such as appendicitis and occult infections in AIDS patients, can be diagnosed within two hours by gamma scintigraphy after i.v. administration of {sup 99m}Tc labeled antibodies reactive with human granulocytes. The antibody, murine IgM anti-SSEA-1, is partially reduced using Sn(II) to expose and protect reactive sulfide groups. The antibody is then purified, stannous tartrate and stabilizers are added, and the mixture is lyophilized. To label, sodium pertechnetate is added. After a 15 minute incubation the tracer drug is injected. The rate of accumulation and degree of concentration at the site of infection is presumptively determinative of the severity of the infection. Acceptance criteria and tests for the {sup 99m}Tc labeled antibody product have been established and validated. Greater than 93% of the {sup 99m}Tc is firmly bound to the protein as determined by quantitative HPLC. Radiochemical impurities, colloidal {sup 99m}Tc and free pertechnetate are together less than 4% as determined by thin layer chromatography. The immunoreactive fraction, measured by binding to solid phase antigen, and affinity measured be ELISA, are unchanged by the {sup 99m}Tc-direct labeling process. Two hour blood clearance in rats is within 90% of the value of the {sup 125}I labeled analog. The immunoreactive fraction decreases less than 10% when incubated in human plasma for 24 hours. This method has been compared to other direct labeling methods, and found to give higher radiochemical yields. 5 figs.

• Research Organization: RhoMed, Inc., Albuquerque, NM (USA)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG04-89ER60899
• OSTI ID: 6055279
• Report Number(s): DOE/ER/60899-5; CONF-9103141-1; ON: DE91009258
• Resource Relation: Conference: Keystone symposium on monoclonal antibodies, Denver, CO (USA), 10-16 Mar 1991
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
ABSCESSES
RADIOIMMUNOSCINTIGRAPHY
INFLAMMATION
MONOCLONAL ANTIBODIES
LABELLING
RADIOPHARMACEUTICALS
DIAGNOSTIC USES
ASCARIS
BIOLOGICAL LOCALIZATION
DIAGNOSIS
GAMMA DETECTION
INDIUM 111
INFECTIOUS DISEASES
LEUKOCYTES
NUCLEAR MEDICINE
PATIENTS
PERFORMANCE TESTING
TECHNETIUM 99
ANTIBODIES
ASCARIDAE
ASCHELMINTHES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DETECTION
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HELMINTHS
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MEDICINE
MINUTES LIVING RADIOISOTOPES
NEMATODES
NUCLEI
ODD-EVEN NUCLEI
PARASITES
PATHOLOGICAL CHANGES
RADIATION DETECTION
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SYMPTOMS
TECHNETIUM ISOTOPES
TESTING
TRACER TECHNIQUES
USES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
400703 - Radiochemistry & Nuclear Chemistry- Radioisotope Production