Inhibition of glycolysis by H sub 2 O sub 2 is not due to inhibition of G3PDH

Conference · Mon Feb 26 00:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6053816

Winkler, B S; Solomon, F J ^[1]

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1. Oakland Univ., Rochester, MI (United States)

The inhibition of glycolysis by H{sub 2}O{sub 2} has been attributed to oxidative inactivation of glyceraldehyde-3-phosphate dehydrogenase (G3PDH). The authors previous work using isolated, intact rat retinas suggested that the inter-action between H{sub 2}O{sub 2} and pyruvate could also contribute to the decrease in glycolysis, though G3PDH was not measured. The strategy of the present work was to compare the effects of H{sub 2}O{sub 2} with those of iodoacetate (IAA), which inactivates G3PDH but does not interact with pyruvate, on glycolysis of retinas and on the activity of G3PDH, as measured in post-incubation supernatant fractions of these retinas. When the enzyme activity was inhibited by 50% with 0.01 mM IAA, the glycolytic rate was not significantly different from the control value. However, when H{sub 2}O{sub 2} produced a 50% inhibition of the enzyme the glycolytic rate was decreased by 70% relative to the control tissue. With 0.1 mM H{sub 2}O{sub 2}, the enzyme inhibition was about 15% but the lactate produced declined by about 20%. Glycolysis was suppressed completely when the enzyme was inhibited by 99+% with either H{sub 2}O{sub 2} or IAA. Though G3PDH is clearly inhibited by H{sub 2}O{sub 2}, it appears that this inhibition alone may not account for the decline in glycolysis.

• OSTI ID: 6053816
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLYCOLYSIS
BIOCHEMICAL REACTION KINETICS
HYDROGEN PEROXIDE
TOXICITY
ENZYME ACTIVITY
INHIBITION
IODINE COMPOUNDS
OXIDOREDUCTASES
RATS
ANIMALS
ENZYMES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
KINETICS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology