Mutagenicity of 3-chlorodibenzofuran and its metabolic activation
- National Inst. for Environmental Studies, Ibaraki (Japan)
3-Chlorodibenzofuran was the only markedly mutagenic isomer among the four monochlorodibenzofurans. Although it was mutagenic even in the absence of 9,000g supernatant fraction (S9) of rat liver, it was further activated by the addition of S9. Metabolic activation of this compound in mutagenicity was studied using liver S9s and cell fractions which were prepared from rats treated with two inducers. 1,1-Dichloro-2,2-bis(p-chlorophenyl) ethylene (DDE) was used as an inducer of phenobarbital inducible cytochrome P-450, and {beta}-naphthoflavone ({beta}NF) was used as an inducer of 3-methylcholanthrene inducible cytochrome P-448. Mutagenicity was tested using Salmonella typhimurium tester strain TA98, because this strain is more sensitive to 3-chlorodibenzofuran than strain TA100. This experiment showed that 3-chlorodibenzofuran was activated most highly by {beta}NF-induced microsomes. However, it was also activated by the cytosolic fraction. This showed that 3-chlorodibenzofuran is activated not only by cytochrome P-448, which is induced by 3-methylcholanthrene type inducers, but also by the enzymes existing in normal rat liver. This result suggests a risk of manifestation of its toxicity to normal animals.
- OSTI ID:
- 6053060
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 17:2; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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BENZOFURANS
METABOLIC ACTIVATION
CHLORINATED AROMATIC HYDROCARBONS
DOSE-RESPONSE RELATIONSHIPS
CYTOCHROMES
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METABOLISM
MICROSOMES
MUTAGEN SCREENING
PHENOBARBITAL
RATS
TOXICITY
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AROMATICS
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BARBITURATES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
FURANS
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HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
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ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
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ORGANS
PIGMENTS
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560300* - Chemicals Metabolism & Toxicology