Accumulation of RNA in blastocysts during embryonic diapause and the periimplantation period in the western spotted skunk

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · J. Exp. Zool.; (United States)

DOI:https://doi.org/10.1002/jez.1402350109· OSTI ID:6045128

Mead, R A; Rourke, A W

The in vivo incorporation of TH-uridine into RNA was studied in delayed implanting and activated blastocysts obtained from 33 western spotted skunks. TH-uridine was incorporated into RNA by all blastocysts; however, significantly more label was incorporated as blastocyst diameter increased. Activated blastocysts with diameters of 1.6 mm or greater on average incorporated 65 times more TH-precursor in 5 hr than diapausing blastocysts with diameters of 1.1 mm or less. Polyadenylated RNA was likewise synthesized by delayed implanting and activated skunk blastocysts; however, the proportion of polyadenylated RNA synthesized by the former was greater than in the latter. The data suggest that the transition from embryonic diapause to fully activated blastocysts first occurs gradually for several days before entering a 1-2-day period of rapid development characterized by an abrupt increase in RNA accumulation.

• Research Organization: Univ. of Idaho, Moscow
• OSTI ID: 6045128
• Journal Information: J. Exp. Zool.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ONTOGENESIS
BIOCHEMISTRY
TRITIUM COMPOUNDS
UPTAKE
URIDINE
KINETICS
PREGNANCY
RNA
RODENTS
ANIMALS
AZINES
CHEMISTRY
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques