Restriction analysis of specific DNA sequences of five homothallic species of Neurospora
Nuclear DNA's from five homothallic species of Neurospora: N. africana, N. dodgei, N. lineolata, N. galapagosensis and N. terricola were isolated and characterized. From these total nuclear DNA's, specific DNA sequences were isolated by CsCl buoyant density gradient equilibrium centrifugation. These selected DNA's were restricted separately with EcoR1, Bam H1, Hind III, XBa 1 and Hinc II. DNA digests were electrophorused in varying agarose gel concentration (0.7%, 0.8% and 1%) and the sizes of fragments generated with these endonucleases were estimated against lambda phage DNA and pMF2 DNA fragments. EcoR1 generated four DNA size variants of about 20.0 kb, 7.5 kb, 5.5 b and 5.23 kb in N. terricola except the 10.5 kb fragment. Instead, N. lineolata showed three distinct DNA size variants of about 15.0 kb, 12.0 kb and 8.2 kb with Bam H1 treatment. Similarly we have noticed unique DNA restriction bands when treated with a few other restriction enzymes like Xba 1 and Hind III. These results suggest distinct rDNA nucleotide sequence differences among homothallic species of Neurospora studied.
- Research Organization:
- Howard Univ., Washington, DC
- OSTI ID:
- 6044778
- Journal Information:
- Genetics; (United States), Vol. 104:1
- Country of Publication:
- United States
- Language:
- English
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