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Title: End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics

Abstract

The role of vitamin D metabolites in the regulation of hepatic 25-hydroxyvitamin D production was investigated by examining the effects of 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D on the synthesis of (25-/sup 3/H)hydroxyvitamin D by rachitic rat liver homogenates. Production of (25-/sup 3/H)hydroxyvitamin D was inhibited by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D, but not by 24,25-dihydroxyvitamin D. 25-Hydroxyvitamin D increased the Km of the vitamin D-25-hydroxylase enzyme(s), while 1,25-dihydroxyvitamin D decreased the Vmax with a Ki of 88.7 ng/ml. Inhibition of hepatic 25-hydroxyvitamin D production by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D may be another control mechanism to regulate circulating vitamin D levels.

Authors:
;
Publication Date:
Research Org.:
Univ. of Massachusetts Medical Center, Worcester
OSTI Identifier:
6037784
Resource Type:
Journal Article
Journal Name:
Arch. Biochem. Biophys.; (United States)
Additional Journal Information:
Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; TRITIUM COMPOUNDS; BIOSYNTHESIS; VITAMIN D; BIOCHEMISTRY; KINETICS; LIVER; RATS; RICKETS; ANIMALS; BODY; CHEMISTRY; DIGESTIVE SYSTEM; DISEASES; GLANDS; LABELLED COMPOUNDS; MAMMALS; METABOLIC DISEASES; ORGANS; RODENTS; SKELETAL DISEASES; SYNTHESIS; VERTEBRATES; VITAMINS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Milne, M L, and Baran, D T. End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics. United States: N. p., 1985. Web. doi:10.1016/0003-9861(85)90234-6.
Milne, M L, & Baran, D T. End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics. United States. https://doi.org/10.1016/0003-9861(85)90234-6
Milne, M L, and Baran, D T. 1985. "End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics". United States. https://doi.org/10.1016/0003-9861(85)90234-6.
@article{osti_6037784,
title = {End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics},
author = {Milne, M L and Baran, D T},
abstractNote = {The role of vitamin D metabolites in the regulation of hepatic 25-hydroxyvitamin D production was investigated by examining the effects of 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D on the synthesis of (25-/sup 3/H)hydroxyvitamin D by rachitic rat liver homogenates. Production of (25-/sup 3/H)hydroxyvitamin D was inhibited by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D, but not by 24,25-dihydroxyvitamin D. 25-Hydroxyvitamin D increased the Km of the vitamin D-25-hydroxylase enzyme(s), while 1,25-dihydroxyvitamin D decreased the Vmax with a Ki of 88.7 ng/ml. Inhibition of hepatic 25-hydroxyvitamin D production by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D may be another control mechanism to regulate circulating vitamin D levels.},
doi = {10.1016/0003-9861(85)90234-6},
url = {https://www.osti.gov/biblio/6037784}, journal = {Arch. Biochem. Biophys.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1985},
month = {Fri Nov 01 00:00:00 EST 1985}
}