Morphologic changes in rat urothelial cells during carcinogenesis. II. Image cytometry
Improved early detection of neoplasia by screening of urothelial cells requires an understanding of the features distinguishing normal and neoplastic cell populations. The authors have begun a program of study based upon a rate model system for the controlled observation of early-stage lesions produced by the carcinogen N-butyl-N-(4-hydroxybutyl)- nitrosamine. Cells dissociated directly from normal and malignant urothelium were characterized by conventional cytopathology techniques and by quantitative microscopy (for nuclear texture and nuclear and cytoplasmic size, shape, and stain content) to derive a comprehensive picture of bladder tumor development. By following the changes that occur in the dissociated urothelial cells the authors have found that the nuclear area, total nuclear stain, nuclear shape, and the nuclear chromatin change significantly over a 48-wk interval as the lesions progress toward malignancy. 24 references, 10 figures, 1 table.
- Research Organization:
- Delft Univ. of Technology, Netherlands
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6037239
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Histochemistry of NADH diaphorase and. gamma. -glutamyltranspeptidase in rat bladder tumors
Inorganic arsenic in drinking water accelerates N-butyl-N-(4-hydroxybutyl)nitrosamine-induced bladder tissue damage in mice
Related Subjects
ANIMAL CELLS
MORPHOLOGICAL CHANGES
BLADDER
EXPERIMENTAL NEOPLASMS
NITROSAMINES
CARCINOGENESIS
BIOLOGICAL MODELS
EXPERIMENTAL DATA
IMAGE PROCESSING
RATS
SCREENING
TOXICITY
AMINES
ANIMALS
BODY
DATA
INFORMATION
MAMMALS
NITROSO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PROCESSING
RODENTS
URINARY TRACT
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)